Extended storage of autologous apheresis platelets in plasma
Article first published online: 6 FEB 2013
© 2013 The Author(s) Vox Sanguinis © 2013 International Society of Blood Transfusion
Volume 104, Issue 4, pages 324–330, May 2013
How to Cite
Slichter, S. J., Bolgiano, D., Corson, J., Jones, M. K., Christoffel, T. and Pellham, E. (2013), Extended storage of autologous apheresis platelets in plasma. Vox Sanguinis, 104: 324–330. doi: 10.1111/vox.12010
- Issue published online: 22 APR 2013
- Article first published online: 6 FEB 2013
- Manuscript Accepted: 2 DEC 2012
- Manuscript Revised: 27 NOV 2012
- Manuscript Received: 27 APR 2012
- National Heart, Lung and Blood Institute
- National Institutes of Health. Grant Number: P50 HL081015
- apheresis platelets;
- platelet storage;
Background and Objectives
The purpose of our studies was to determine the effects of extended platelet storage on poststorage platelet viability.
Materials and Methods
Normal subjects were recruited to donate platelets using two different apheresis systems: either the COBE Spectra (n = 58) or the Haemonetics MCS+ (n = 84). Platelet recovery and survival data from the two systems were compared with each other and with in vitro measurements of the stored platelets.
There were no significant differences in either platelet recoveries or survivals between the two machines between 1 and 8 days of storage. Combining the data from both machines, platelet recoveries decreased by 2·6% and survivals by 0·3 days/storage day. In vitro assays did not predict either platelet recoveries or survivals during storage for 5–8 days. After 9 days of storage, pHs were unacceptable (≤ 6·1), suggesting that 8 days will be the longest possible storage time.
These data suggest that, if stored platelet bacterial contamination issues are resolved, significant extension of platelet storage times is possible.