Growth factor release from platelet concentrates: analytic quantification and characterization for clinical applications

Authors

  • C. Durante,

    1. Dipartimento di Ricerca Traslazionale, S.O.S. Dipartimentale Trattamento Cellule Staminali per le Terapie Cellulari, Centro di Riferimento Oncologico, IRCCS, Aviano, PN, Italy
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    • These authors contributed equally to this study.
  • F. Agostini,

    1. Dipartimento di Ricerca Traslazionale, S.O.S. Dipartimentale Trattamento Cellule Staminali per le Terapie Cellulari, Centro di Riferimento Oncologico, IRCCS, Aviano, PN, Italy
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    • These authors contributed equally to this study.
  • L. Abbruzzese,

    1. Dipartimento di Ricerca Traslazionale, S.O.S. Dipartimentale Trattamento Cellule Staminali per le Terapie Cellulari, Centro di Riferimento Oncologico, IRCCS, Aviano, PN, Italy
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  • R. T. Toffola,

    1. Dipartimento di Ricerca Traslazionale, S.O.S. Dipartimentale Trattamento Cellule Staminali per le Terapie Cellulari, Centro di Riferimento Oncologico, IRCCS, Aviano, PN, Italy
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  • S. Zanolin,

    1. Dipartimento di Ricerca Traslazionale, S.O.S. Dipartimentale Trattamento Cellule Staminali per le Terapie Cellulari, Centro di Riferimento Oncologico, IRCCS, Aviano, PN, Italy
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  • C. Suine,

    1. Dipartimento di Ricerca Traslazionale, S.O.S. Dipartimentale Trattamento Cellule Staminali per le Terapie Cellulari, Centro di Riferimento Oncologico, IRCCS, Aviano, PN, Italy
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  • M. Mazzucato

    Corresponding author
    • Dipartimento di Ricerca Traslazionale, S.O.S. Dipartimentale Trattamento Cellule Staminali per le Terapie Cellulari, Centro di Riferimento Oncologico, IRCCS, Aviano, PN, Italy
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Correspondence: Dr. Mario Mazzucato, Centro di Riferimento Oncologico, IRCCS, Via Franco Gallini 2, 33081 Aviano, PN, Italy

E-mail: mmazzucato@cro.it

Abstract

Background and Objectives

Clinical use of plasma rich in growth factors requires biochemical product control. We aimed to measure and modulate concentrations of growth factors in solutions deriving from platelet apheresis or whole blood.

Materials and Methods

Growth factor concentrations were measured 5’, 10’, 20’, 30’, 60’ after CaCl2 was added at 40°C to platelet-apheresis products (= 39) or after 60’ in platelet concentrates from whole blood (= 13). Growth factor release was also obtained in platelet apheresis a) by incubation at 22°C or 40°C for 10’ or 30’ (= 4); b) by repeated freeze–thaw (= 9).

Results

Fibroblast growth factor (FGF), platelet-derived growth factor (PDGF) isoforms AA and AB and transforming growth factor beta (TGF-β) concentrations (pg/10plt) were 25–60% higher in growth factors solutions from whole blood compared to platelet apheresis. Vascular endothelial growth factor (VEGF), TGF-β and PDGF isoforms were released early (5–10’) during incubation: TGF-β concentration increased also at 30’. FGF and epidermal growth factor (EGF) were released only after 30’. Incubation at 40°C/10’ increased VEGF (+70%) and decreased EGF (−30%) and PDGF-BB (−50%) versus 22°C/30’. Shock significantly increased TGF-β (1·6-fold), EGF (1·5-fold), FGF (4·5-fold) and lowered PDGF isoforms (0·2- to 0·5-fold) versus prolonged incubation at 40°C.

Conclusion

Platelets from platelet apheresis and whole-blood release all investigated growth factors. The release can be regulated controlling incubation time and/or temperature and performing cell lysis.

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