Renal dual-phase computed tomograpic angiography (CTA) is used to assess suitability of feline donors prior to transplantation. A prerequisite for successful CTA is optimal synchronization between the arterial passage of contrast material and CT data acquisition. This retrospective study was conducted to compare quality of renal vascular enhancement at dual-phase CTA in normal cats between two techniques of timing of data acquisition: the timing-bolus and the bolus tracking method. Nine cats were scanned using the timing-bolus technique and 14 with the bolus tracking technique using otherwise similar scanning parameters in a 16-slice multidetector row CT scanner. The quality of enhancement of the renal vessels at the scanned arterial phase and venous phase was assessed both subjectively and objectively by three board-certified radiologists. Arterial enhancement was not observed at the scanned arterial phase in three of the nine cats with the timing-bolus technique but only 1 of the 14 cats with the bolus tracking technique. Early venous enhancement at the scanned arterial phase was common with the bolus tracking technique. Data acquisition was significantly faster with the bolus tracking technique. We conclude that the bolus tracking technique is a valid technique that could be integrated into the routine protocol for 16-detector row CT renal angiography in cats.