A 4-year-old, spayed female greyhound dog was presented with an acute onset of paraplegia. There was no known history of trauma or coagulopathy. Spinal cord compression was identified on MRI. Intra-operative evaluation revealed the presence of a large subperiosteal hematoma and a smaller epidural hematoma. To the authors’ knowledge, this is the first report of a spinal subperiosteal hematoma diagnosed antemortem through MRI, with surgical exploration and successful treatment in a dog.