Original Research-Basic Science
Whole animal knockout of smooth muscle alpha-actin does not alter excisional wound healing or the fibroblast-to-myofibroblast transition
Article first published online: 18 DEC 2012
© 2012 by the Wound Healing Society
Wound Repair and Regeneration
Volume 21, Issue 1, pages 166–176, January-February 2013
How to Cite
Tomasek, J. J., Haaksma, C. J., Schwartz, R. J. and Howard, E. W. (2013), Whole animal knockout of smooth muscle alpha-actin does not alter excisional wound healing or the fibroblast-to-myofibroblast transition. Wound Repair and Regeneration, 21: 166–176. doi: 10.1111/wrr.12001
- Issue published online: 8 JAN 2013
- Article first published online: 18 DEC 2012
- Manuscript Accepted: 15 OCT 2012
- Manuscript Received: 9 FEB 2012
- National Institutes of Health. Grant Number: R01 GM060651
Figure S1. SMαA is not expressed in dermis and granulation tissue of SMαA-null mice. Dermis and day 12 granulation tissue was collected from WT and SMαA-null mice and analyzed by semi-quantitative RT-PCR for SMαA. SMαA expression increased in WT day 12 granulation tissue (WT-W) compared with WT dermis (WT-D). No SMαA expression was observed in SMαA-null dermis (Null-D) or day 12 granulation tissue (Null-W). RNA level was normalized to GAPDH.
Figure S2. Higher magnification images of hematoxylin and eosin stained sections of 12-day granulation tissue from WT (A) and SMαA-null (B) mice. No major differences in histological appearance between the WT and SMαA-null granulation tissue were observed; b = 50 μm.
Figure S3. Tandem mass spectrometry analysis for expression by WT (A,C,E,G,I,K,M,O,Q,S,U) and SMαA-null myofibroblasts (B,D,F,H,J,L,N,P,R,T,V) for cytoplasmic β-actin (C–F), cytoplasmic γ-actin (G–J), SMαA (K–N), SMγA (O–R), SkMαA (S–V).
Table S1. Sequences of oligonucleotide primers used for semi-quantitative RT-PCR and for real-time RT-PCR.
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