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Figure S1. SMαA is not expressed in dermis and granulation tissue of SMαA-null mice. Dermis and day 12 granulation tissue was collected from WT and SMαA-null mice and analyzed by semi-quantitative RT-PCR for SMαA. SMαA expression increased in WT day 12 granulation tissue (WT-W) compared with WT dermis (WT-D). No SMαA expression was observed in SMαA-null dermis (Null-D) or day 12 granulation tissue (Null-W). RNA level was normalized to GAPDH.

Figure S2. Higher magnification images of hematoxylin and eosin stained sections of 12-day granulation tissue from WT (A) and SMαA-null (B) mice. No major differences in histological appearance between the WT and SMαA-null granulation tissue were observed; b = 50 μm.

Figure S3. Tandem mass spectrometry analysis for expression by WT (A,C,E,G,I,K,M,O,Q,S,U) and SMαA-null myofibroblasts (B,D,F,H,J,L,N,P,R,T,V) for cytoplasmic β-actin (C–F), cytoplasmic γ-actin (G–J), SMαA (K–N), SMγA (O–R), SkMαA (S–V).

Table S1. Sequences of oligonucleotide primers used for semi-quantitative RT-PCR and for real-time RT-PCR.

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