Present address: Department of Surgery, Institute for Cellular Transplantation, College of Medicine, University of Arizona, PO Box 245066, Tucson, AZ, USA.
Real-time assessment of encapsulated neonatal porcine islets prior to clinical xenotransplantation
Article first published online: 23 OCT 2012
© 2012 John Wiley & Sons A/S
Volume 19, Issue 6, pages 333–336, November/December 2012
How to Cite
Kitzmann, J. P., Law, L., Shome, A., Muzina, M., Elliott, R. B., Mueller, K. R., Schuurman, H.-J. and Papas, K. K. (2012), Real-time assessment of encapsulated neonatal porcine islets prior to clinical xenotransplantation. Xenotransplantation, 19: 333–336. doi: 10.1111/xen.12005
- Issue published online: 2 DEC 2012
- Article first published online: 23 OCT 2012
- Received 26 June 2012; Accepted 18 September 2012
- functional assessment;
- oxygen consumption rate;
Kitzmann JP, Law L, Shome A, Muzina M, Elliott RB, Mueller KR, Schuurman H-J, Papas KK. Real-time assessment of encapsulated neonatal porcine islets prior to clinical xenotransplantation. Xenotransplantation 2012; 19: 333–336. © 2012 John Wiley & Sons A/S.
Abstract: Background: Porcine islet transplantation is emerging as an attractive option for the treatment of patients with type 1 diabetes, with the possibility of providing islets of higher and more consistent quality and in larger volumes than available from human pancreata. The use of encapsulated neonatal porcine islets (ENPI) is appealing because it can address islet supply limitations while reducing the need for anti-rejection therapy. Pre-transplant characterization of ENPI viability and potency is an essential component of the production process. We applied the validated assay for oxygen consumption rate normalized for DNA content (OCR/DNA) to characterize ENPI viability.
Methods: ENPI of low viscosity and high m alginate were prepared according to standard methods and characterized at various culture time points up to 5 weeks.
Results: The OCR/DNA (nmol/min·mgDNA ± SEM) of ENPI (235 ± 10, n = 9) was comparable to that of free NPI (255 ± 14, n = 13). After encapsulation, NPI OCR/DNA was sustained over a culture period of up to 5 weeks. The average OCR/DNA of ENPI cultured longer than 9 days was higher than that of freshly encapsulated NPI.
Conclusion: This is the first characterization of ENPI by a validated and more sensitive method for product viability. The NPI encapsulation process does not compromise viability as measured by OCR/DNA, and ENPI can be cultured for up to 5 weeks with maintenance of viability. ENPI meet or exceed current adult porcine islet product release criteria (established at the University of Minnesota) for preclinical xenotransplantation in terms of OCR/DNA.