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Phylogeny, phylogeography, and systematics of the American pea crab genus Calyptraeotheres Campos, 1990, inferred from molecular markers

Authors

  • Emiliano H. Ocampo,

    Corresponding author
    • Instituto de Investigaciones Marinas y Costeras, CONICET-UNMDP, Mar del Plata, Buenos Aires, Argentina
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  • Rafael Robles,

    1. Laboratory of Bioecology and Crustacean Systematics, Program in Comparative Biology, Department of Biology, Faculty of Philosophy, Science and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
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  • Mariana Terossi,

    1. Laboratory of Bioecology and Crustacean Systematics, Program in Comparative Biology, Department of Biology, Faculty of Philosophy, Science and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
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  • Jesús D. Nuñez,

    1. Instituto de Investigaciones Marinas y Costeras, CONICET-UNMDP, Mar del Plata, Buenos Aires, Argentina
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  • Maximiliano Cledón,

    1. Instituto de Investigaciones Marinas y Costeras, CONICET-UNMDP, Mar del Plata, Buenos Aires, Argentina
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  • Fernando L. Mantelatto

    1. Laboratory of Bioecology and Crustacean Systematics, Program in Comparative Biology, Department of Biology, Faculty of Philosophy, Science and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
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Corresponding author. E-mail: eocampo@mdp.edu.ar

Abstract

We used mitochondrial cytochrome oxidase I (COI) and the large ribosomal subunit (16S) genes to establish evolutionary relationships amongst species of Calyptraeotheres, evaluate their usefulness as DNA-barcoding genes, and assess molecular diversity at the population level within Calyptraeotheres garthi. Bayesian, maximum likelihood, and maximum parsimony phylogenies confirmed the monophyly of Calyptraeotheres, showing that the ancestor of C. garthi, Calyptraeotheres hernandezi, and Calyptraeotheres granti radiated after the formation of the Panamanian isthmus. This finding contradicts the austral/tropical hypothesis previously proposed based on morphological data. The COI and 16S distance matrices supported separation of species as well as the genera, and corroborated that DNA barcoding is a useful tool and complements the classical taxonomy in Pinnotheridae. Phylogenetic and genetic distance analyses suggested that C. hernandezi is a junior synonym of C. garthi. Finally, C. garthi did not show a population structure across its distribution range, and showed a pattern consistent with a recent population expansion event that began 230–300 Kya. © 2013 The Linnean Society of London

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