Anion permeability of the inhibitory post-synaptic membrane of the crayfish neuromuscular junction
Article first published online: 1 AUG 1967
© 1967 The Physiological Society
The Journal of Physiology
Volume 191, Issue 3, pages 575–590, August 1, 1967
How to Cite
1967), Anion permeability of the inhibitory post-synaptic membrane of the crayfish neuromuscular junction. The Journal of Physiology, 191 doi: 10.1113/jphysiol.1967.sp008269., , (
- Issue published online: 1 AUG 1967
- Article first published online: 1 AUG 1967
- Cited By
1. The membrane conductance of the crayfish muscle fibre was calculated and the increase in the membrane conductance brought about by the application of γ-aminobutyric acid (GABA) was investigated under various conditions. The permeability of the activated inhibitory post-synaptic membrane was estimated from the conductance change.
2. When the concentration of Cl− was changed by substituting Cl− with methylsulphate, a linear relation was obtained between the membrane conductance change produced by GABA and the concentration of Cl−. Cations have no appreciable effect on the GABA induced conductance change (less than 0·4%).
3. The conductance change produced by GABA on the inhibitory post-synaptic membrane was measured in various solutions in which the Cl− was replaced by various anions. The degree of permeability depended on the anion species in the following order:
Br− > Cl− > SCN− > I− > NO3− > HCOO− > ClO3− > ClO4− > BrO3−
4. At high pH the conductance change produced by GABA was decreased, while at low pH it increased.
5. These results show that the activated inhibitory post-synaptic membrane of crayfish muscle fibres has the characteristics of an anion selective membrane, suggesting the fixed positive charges in the inhibitory membrane.