Calcium-dependent inactivation of heteromeric NMDA receptor-channels expressed in human embryonic kidney cells.
Article first published online: 1 FEB 1995
© 1995 The Physiological Society
The Journal of Physiology
Volume 482, Issue 3, pages 567–573, February 1, 1995
How to Cite
1995), Calcium-dependent inactivation of heteromeric NMDA receptor-channels expressed in human embryonic kidney cells.. The Journal of Physiology, 482 doi: 10.1113/jphysiol.1995.sp020540., , , , , , , (
- Issue published online: 1 FEB 1995
- Article first published online: 1 FEB 1995
- Cited By
1. Whole-cell current through heteromeric NR1-NR2A and NR1-NR2B subunit combinations of NMDA channels transiently expressed in human embryonic kidney cells (HEK 293) were studied using the patch-clamp technique. 2. With 4 mM Mg-ATP in the internal pipette solution, the responses of cells expressing NR1-NR2A channels to glutamate application gradually decreased, reaching 50% of control during the first 20 min of recording. This process was accompanied by acceleration of desensitization. 3. Conditioning (5-15 s) applications of glutamate (100 microM) induced a transient inactivation of NR1-NR2A and NR1-NR2B channels (20-40%) with a slow time course of recovery (tau r = 10-60 s). Both the degree of inactivation and the time constant of recovery increased with the duration of conditioning applications of glutamate, and with an elevation of Ca2+ in the external solution. 4. These results show that both NR1-NR2A and NR1-NR2B recombinant NMDA receptor-channels expressed in HEK 293 cells can be transiently inhibited by Ca2+ ions in a similar way to that described for hippocampal neurones.