Author's present address M. Dutschmann: Department of Animal Physiology, University of Tübingen, Auf der Morgenstelle 28, 72076 Tübingen, Germany.
Glycinergic inhibition is essential for co-ordinating cranial and spinal respiratory motor outputs in the neonatal rat
Article first published online: 22 JUL 2004
The Journal of Physiology
Volume 543, Issue 2, pages 643–653, September 2002
How to Cite
Dutschmann, M. and Paton, J. F. R. (2002), Glycinergic inhibition is essential for co-ordinating cranial and spinal respiratory motor outputs in the neonatal rat. The Journal of Physiology, 543: 643–653. doi: 10.1113/jphysiol.2001.013466
- Issue published online: 22 JUL 2004
- Article first published online: 22 JUL 2004
- (Received 30 October 2001; accepted after revision 10 June 2002)
Eupnoeic breathing in mammals is dependent on the co-ordinated activity of cranial and spinal motor outputs to both ventilate the lungs and adjust respiratory airflow, which they do by regulating upper-airway resistance. We investigated the role of central glycinergic inhibition in the co-ordination of cranial and spinal respiratory motor outflows. We developed an arterially perfused neonatal rat preparation (postnatal age 0–4 days) to assess the effects of blocking glycine receptors with systemically administered strychnine (0.5–1 μM). We recorded respiratory neurones located within the ventrolateral medulla, inspiratory phrenic nerve activity (PNA) and recurrent laryngeal nerve activity (RLNA), as well as dynamic changes in laryngeal resistance. Central recordings of postinspiratory neurones revealed an earlier onset in firing relative to the onset of inspiratory PNA after exposure to strychnine (260 ± 38.9 vs. 129 ± 26.8 ms). After glycine receptor blockade, postinspiratory neurones discharged during the inspiratory phase. Strychnine also evoked a decrease in PNA frequency (from 38.6 ± 4.7 to 30.7 ± 2.8 bursts min−1), but amplitude was unaffected. In control conditions, RLNA comprised inspiratory and postinspiratory discharges; the amplitude of the latter exceeded that of the former. However, after administration of strychnine, the amplitude of inspiratory-related discharge increased (+65.2 ± 15.2%) and exceeded postinspiratory activity. Functionally this change in RLNA caused a paradoxical, inspiratory-related glottal constriction during PNA. We conclude that during the first days of life in the rat, glycine receptors are essential for the formation of the eupnoeic-like breathing pattern as defined by the co-ordinated activity of cranial and spinal motor inspiratory and postinspiratory activities.