S. Göpel and Q. Zhang contributed equally to this study and their names appear in alphabetical order.
Capacitance measurements of exocytosis in mouse pancreatic α-, β- and δ-cells within intact islets of Langerhans
Article first published online: 21 APR 2004
The Journal of Physiology
Volume 556, Issue 3, pages 711–726, May 2004
How to Cite
Göpel, S., Zhang, Q., Eliasson, L., Ma, X.-S., Galvanovskis, J., Kanno, T., Salehi, A. and Rorsman, P. (2004), Capacitance measurements of exocytosis in mouse pancreatic α-, β- and δ-cells within intact islets of Langerhans. The Journal of Physiology, 556: 711–726. doi: 10.1113/jphysiol.2003.059675
- Issue published online: 21 APR 2004
- Article first published online: 21 APR 2004
- (Received 14 December 2003; accepted after revision 6 February 2004; first published online 13 February 2004)
Capacitance measurements of exocytosis were applied to functionally identified α-, β- and δ-cells in intact mouse pancreatic islets. The maximum rate of capacitance increase in β-cells during a depolarization to 0 mV was equivalent to 14 granules s−1, <5% of that observed in isolated β-cells. β-Cell secretion exhibited bell-shaped voltage dependence and peaked at +20 mV. At physiological membrane potentials (up to ∼−20 mV) the maximum rate of release was ∼4 granules s−1. Both exocytosis (measured by capacitance measurements) and insulin release (detected by radioimmunoassay) were strongly inhibited by the L-type Ca2+ channel blocker nifedipine (25 μm) but only marginally (<20%) affected by the R-type Ca2+ channel blocker SNX482 (100 nm). Exocytosis in the glucagon-producing α-cells peaked at +20 mV. The capacitance increases elicited by pulses to 0 mV exhibited biphasic kinetics and consisted of an initial transient (150 granules s−1) and a sustained late component (30 granules s−1). Whereas addition of the N-type Ca2+ channel blocker ω-conotoxin GVIA (0.1 μm) inhibited glucagon secretion measured in the presence of 1 mm glucose to the same extent as an elevation of glucose to 20 mm, the L-type Ca2+ channel blocker nifedipine (25 μm) had no effect. Thus, glucagon release during hyperglycaemic conditions depends principally on Ca2+-influx through N-type rather than L-type Ca2+ channels. Exocytosis in the somatostatin-secreting δ-cells likewise exhibited two kinetically separable phases of capacitance increase and consisted of an early rapid (600 granules s−1) component followed by a sustained slower (60 granules s−1) component. We conclude that (1) capacitance measurements in intact pancreatic islets are feasible; (2) exocytosis measured in β-cells in situ is significantly slower than that of isolated cells; and (3) the different types of islet cells exhibit distinct exocytotic features.