Glucocorticoids and insulin both modulate caloric intake through actions on the brain

Authors


  • This report was presented at The Journal of Physiology Symposium on Obesity and the central nervous system, Washington, DC, USA, 1 September 2007. It was commissioned by the Editorial Board and reflects the views of the author.

Corresponding author M. Dallman: Department of Physiology, Box 0444, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143-0444, USA. Email: mary.dallman@ucsf.edu

Abstract

Glucocorticoids act primarily in a feed-forward fashion on brain to activate CNS pathways that implement wanting appropriate to physiological needs. Thus, depending on the available conditions, elevated glucocorticoids may augment the behavioural want to run, fight or feed. Although glucocorticoids stimulate intake of chow, fat and sucrose, insulin appears to sculpt calorie-associated desires toward foods high in fat, acting through hepatic branch afferents of the vagus nerve. Both conditions of reduced food allowance and chronic stress excite glucocorticoid-augmented central neural networks that may lead toward ultimate abdominal obesity.

Adrenal glucocorticoids (GC) are well known to mobilize substrate from peripheral energy depots such as muscle and fat for use in hepatic gluconeogenesis, insuring a plentiful supply of glucose for use under conditions of challenge when flight or fight may be necessary. However, GC also have marked and complementary effects on the brain, that serve to augment behaviours, autonomic and neuroendocrine outflows, and learning and memory that are particularly associated with body energy balance and maintenance of life during challenging periods. During the past decade our lab has been exploring the roles of energy stores, GC and insulin on feeding behaviours and central stress responses (Pecoraro et al. 2006).

Below we review studies that show when adrenalectomized (ADX) rats are provided with high-density sucrose solutions, the rats normalize caloric stores and central corticotropin-releasing factor (CRF) expression to those levels that are observed in sham-operated animals. Drinking the pleasurable, high density calories restores neuroendocrine, autonomic outflows and energy stores to normal in rats without GC. This finding suggested a new working model for feedback in the hypothalamo-pituitary adrenal (HPA) axis that we have since tested. Ingestion of sweet (sucrose and saccharin) and fat (lard) substances, and searching for rewarding food, and memory for it, is proportional to the circulating GC environment, again invoking the powerful effects of GC on shaping behaviours associated with feeding. GC also stimulate insulin secretion, and we have found that it is the interaction between GC and insulin that modulates the choice of fat (lard) intake. The action of insulin is on the liver, probably through insulin receptors, and mediated through hepatic branch vagal afferents to the brain to provoke lard intake. In the absence of insulin, lard eating does not persist beyond one day in diabetic rats. Thus, through a variety of different mechanisms, the GC insure caloric intake particularly intake of high-density, pleasurable calories. In the presence of chronic stressors, this type of feeding may become habitual. If increased intake of comfort foods does become a habit, abdominal obesity may result, leading to many of the current ills of our society (Dallman et al. 2007).

Adrenalectomy and sucrose: a new model of feedback regulation in the HPA axis (Fig. 1)

Figure 1.

New working model for regulation of activity in the HPA axis
The schema on the left shows the standard GC-mediated feedback of HPA function on the brain as well as the fact that the GC act on peripheral energy stores. The schema on the right shows our current working model. Note that the effect of GC on brain is now excitatory, and that there is a signal from energy stores that now is inhibitory on the brain and HPA axis (GC, glucocorticoids; CNS, central nervous system; CRF, corticotropin-releasing factor; MA, monoamines; DA, dopamine).

When ADX rats are given saline to drink (thus preventing sodium depletion due to loss of aldosterone) and chow to eat ad libitum, there is a constellation of metabolic consequences that occurs in the absence of GC. Male rats eat slightly less than normal, increase body weight at slower rates, have decreased fat mass and increased general sympathetic tone; all of these effects are corrected by supplying corticosterone (Akana et al. 1985; Dallman et al. 2003a). However, to our great surprise, we also found that allowing ADX rats to drink a solution of 32% sucrose, as well as chow and saline ad libitum in the absence of corticosterone, prevented the metabolic deficiencies from occurring (Bell et al. 2000; Laugero et al. 2001). Providing sucrose as well as saline to drink restored thermogenesis (as measured by uncoupling protein-1) to normal, and thus appeared to reduce sympathetic outflow induced by ADX (Bell et al. 2000). Moreover, voluntary sucrose drinking also prevented the well-known changes in central CRF that normally occur both in the hypothalamus and amygdala after ADX (Fig. 2).

Figure 2.

Sucrose ingestion is inversely related to CRF mRNA in the paraventricular nuclei (PVN)
In bilaterally adrenalectomized rats, the total sucrose intake during the 9 days it was available is tightly related to the expression of hypothalamic CRF. CRF mRNA was restored to values similar to those in sham-operated rats (5 points on the right) reducing the normally elevated CRF seen in adrenalectomized rats (4 points on the left) to values similar to rats with intact adrenals (data from Laugero et al. 2001).

When trying to understand how sucrose drinking had such marked effects not only peripherally, but also on brain CRF, we found that there was a quite strong correlation (r=−0.64) with mesenteric fat mass (Dallman et al. 2003b), and we suggested the new model of feedback regulation of HPA axis function shown in Fig. 1. It appears that the brain is informed of fat storage, particularly in the mesenteric fat, and that this information reduces activity in central CRF systems. The limbic CRF system appears to be recruited by chronic stressors, and represents a critical component of the chronic stress response system (Dallman et al. 2006).

We have tested this hypothesis by providing sucrose to adrenalectomized rats replaced with corticosterone and subjected to cold. Under conditions of low, clamped corticosterone concentrations, sucrose, and increased fat mass, were important in diminishing the central responses and increasing thermogenic responses to cold (Bell et al. 2002). Although we hypothesize that there is neural feedback from fat depots to the central nervous system, this has not been identified, yet. Nonetheless, it seems clear in intact animals, as well, that increasing energy stores in the form of fat depot weight, reduces central neural responses to either acute (la Fleur et al. 2005a), or chronic (Pecoraro et al. 2004) stressors.

Some actions of glucocorticoids on brain and feeding behaviour

GC infused directly into the brain ventricular system of ADX rats excite, rather than inhibit both hypothalamic CRF and pituitary adrenocorticotropin hormone (ACTH) secretion, and they also appear to negate the effect of drinking sucrose on metabolism in these rats (Laugero et al. 2002). Thus, in brain, the GC appear to act on the HPA axis in a feed-forward, rather than in the canonical feedback fashion that is usually envisioned (see Fig. 1).

In keeping with central stimulation of CRF, the GC also stimulate behaviours associated with nutrient gain (Dallman et al. 2005). The GC appear to stimulate ongoing behaviour that is dependent on the context, and simply seem to intensify the drive to perform the behaviour; this may well be a consequence of the effects of GC on dopamine secretion in the shell of the nucleus accumbens, the so-called pleasure centre (Barrot et al. 2000). Fighting (Haller et al. 2000; Mikics et al. 2004), risk assessment (Mikics et al. 2005) search (Pecoraro et al. 2005) and running behaviours (Leshner, 1971) are all augmented by the adrenal steroids, as is feeding behaviour.

Glucocorticoids increase the voluntary intake of palatable foods in a dose-related manner (Bell et al. 2000; Bhatnagar et al. 2000; la Fleur et al. 2004); however, they do not stimulate chow intake in the presence of normal insulin concentrations (Strack et al. 1995; la Fleur et al. 2004). When ADX rats are provided with corticosterone replacement that results in steady-state concentrations in plasma, insulin concentrations increase pari passu with corticosterone (Akana et al. 1985; Strack et al. 1995; Bell et al. 2000), Together, these hormones act to increase fat storage, particularly mesenteric fat, although with high GC there is still marked peripheral catabolism and a decreased rate of body weight gain. (Fig. 3).

Figure 3.

Corticosterone has opposite effects on body weight gain and mesenteric fat weight
In adrenalectomized, corticosterone-treated rats allowed sucrose to drink ad libitum, body weight decreases (left panel) as mesenteric fat weight increases (right panel) showing a central shift of calorie storage (data from Bell et al. 2000).

Examining the streptozotocin-diabetic rat, la Fleur showed clearly that ADX rats ate increasing amounts of chow with increasing corticosterone, but that they would not eat lard, unless they were also infused with insulin that provided low circulating concentrations (la Fleur et al. 2004). Of course, increasing GC stimulates increasing concentrations of insulin in the circulation (Fig. 4), and it may be this action of the GC that indirectly results in increased intake of pleasurable foods. The GC may provide the wanting for calories, but the increased insulin may determine what food is wanted in conditions of choice. In her studies, la Fleur also showed that specific hepatic vagal afferents to hypothalamus and amygdala were involved in insulin-induced lard intake in diabetic rats (la Fleur et al. 2003, 2005b).

Figure 4.

As corticosterone increases, circulating insulin increases
In adrenalectomized, corticosterone-treated rats allowed lard to eat ad libitum, plasma insulin increased with steady-state corticosterone concentrations. This also occurs in similarly treated rats allowed only chow, but the extent of the effect is greater when the rats are also given the choice of lard to eat (data from la Fleur et al. 2004).

Actions of insulin and the hepatic branch of the vagus nerve on lard intake

We continued to study where insulin acts to stimulate lard intake in streptozotocin-diabetic rats given a subcutaneous pellet of corticosterone that produced steady-state plasma concentrations that were slightly below the circadian maximum. Such corticosterone replacement blocks endogenous ACTH and adrenal secretion in the absence of chronic stressors (Akana et al. 1992) and stimulates high caloric intake of either chow or lard, depending on the circulating insulin concentrations (la Fleur et al. 2004).

In the first study, we compared the effects of insulin infusions into the jugular versus the superior mesenteric veins (Warne et al. 2006). With 5 days of ad libitum lard availability, both groups of insulin-infused rats ate roughly the same amount of lard as non-diabetic controls, although the vehicle-infused rats ate very little lard. Moreover, all of the diabetic rats were equally hyperphagic, although the insulin infusion resulted in decreased chow intake, compensating for the increased calories ingested as lard. Although the insulin infusions decreased circulating glucose somewhat, because the dose was low (3 U day−1), all diabetic rats were markedly hyperglycaemic. However, the jugular insulin infusion restored subcutaneous fat depot weight to normal and increased body weight markedly whereas the mesenteric insulin infusion restored mesenteric fat depot weight without increasing body weight (Warne et al. 2006). Both sites of insulin infusion reinstated lard ingestion and total intake was regulated, but the infusion site clearly determined the metabolic outcome.

Next, we compared c-Fos immunoreactivity in brains of rats that were made diabetic, given corticosterone and infused into the jugular or superior mesenteric vein with either saline or insulin. In order to obtain an acute lard-feeding stimulus, we remove lard but not chow for 8 h on the fifth day and restored it for an hour in the dark activity period before collecting brains. The results showed two patterns of c-Fos staining: one associated with circulating insulin concentrations which were the same in the insulin-infused groups, and the other associated with a large amount of lard ingestion that was only observed in the rats infused with insulin into the mesenteric vein. The lard-associated c-Fos patterns suggested that the known opiatergic ‘pleasure’ network from the nucleus of the tractus solitarius throughout the brain, including the ventral tegmental area, n. accumbens and other limbic memory sites were affected by eating appreciable amounts of lard (Warne et al. 2007b). These results suggested strongly that a vagal afferent signal stimulated by insulin accompanies lard eating.

In subsequent studies that are still ongoing, we have attempted to differentiate among hepatic branch vagal afferent and efferent neural actions that might mediate the effects of insulin. Figure 5 shows a schematic design of our manipulations. Cutting the hepatic branch of the vagus (Warne et al. 2007a) restored lard intake to normal in diabetic rats without insulin, suggesting strongly that normally, in the absence of insulin, the hepatic branch of the vagus inhibits the ingestion of lard. Infusion of insulin, however, over-rides the inhibitory signal. The results of treating the hepatic branch vagus with capsaicin to denervate hepatic afferent input to brain are detailed in this special issue of The Journal of Physiology (Warne et al.).

Figure 5.

Outline of paradigm to determine how the hepatic branch vagus modulates lard intake
In published and ongoing experiments we are testing the role of the hepatic branch of the vagus (which includes afferents and efferents to both the liver and gastroduodenum), the gastroduodenal branch of the hepatic vagus, and the role of afferent nerves from both sites. The anatomy is indicated for the part of the vagus nerve of interest. Scissors and dotted lines denote severing the nerve; the x indicates the site of painting the nerve with capsaicin in an attempt to destroy afferent fibres without impairing the efferents (experiments of Warne et al. 2006).

We are continuing these studies with the anticipation that we will be able to evolve our understanding of the role(s) of the hepatic branch of the vagus nerve, both the afferent and efferent components, as well as the hepatic actions of insulin, on the drive of rats to voluntarily ingest lard. There were marked alterations in brain c-Fos patterns of expression, and we anticipate altered expression of hypothalamic and limbic brain neuromodulators as a consequence of eating lard and the various vagal manoeuvers, as was found under slightly different conditions after hepatic branch vagotomy (la Fleur et al. 2005b).

Conclusions

GC and insulin are intimately entwined in regulating both the amount and choice of food intake and caloric disposition (Dallman et al. 1993). Both act in the periphery and the central nervous system, generally in opposing directions. GC act centrally to motivate caloric intake and the learning, memories and behaviours associated with this, although they may induce neuronal apoptosis (Sapolsky, 1992). By contrast, insulin acts at the hypothalamus (Woods & Porte, 1983) as well as on the reward system (Figlewicz et al. 2004, 2006) to limit caloric intake. Insulin, like GC, promotes learning and memory but is anti-apoptotic (van der Heide et al. 2006). Peripherally, GC reduce calories stored in fat and protein and increase insulin, whereas insulin increases calories stored in both sites (Leibel et al. 1989) and also seems to act systemically through over-riding a normal inhibitory hepatic signal to promote the ingestion of lard calories (Warne et al. 2007a). Together these hormones synergize to greatly expand mesenteric fat depots, putting stored calories in a site that is highly labile and where fat can be readily mobilized by the sympathetic nervous system for immediate use by the liver.

These actions of GC and insulin are obviously highly evolutionarily useful, allowing organisms to respond to and have the stored energy available to escape threat. However, today's wealthy societies may have outstripped this usefulness for the evolved actions of these hormones. Life in the 21st century is fast-paced, food is abundant and readily available; furthermore, there is a perception that persistent stressors are increasing and may lead to stress-associated diseases (Rozanski et al. 1999). Tellingly, a recent report shows prospective evidence that job strain and poor social support is significantly associated with increased body mass index and abdominal obesity (Brunner et al. 2007). It may be that the evolutionarily key interactions between the stress hormones and insulin are now our undoing, given the current environment.

Appendix

Acknowledgements

We are very grateful for the help generously given by Drs Susan F Akana, Abigail Ginsberg and Ms Hart Horneman in realizing the results of many of the experiments discussed. We also are pleased to acknowledge that the work was supported by grants from the National Institutes of Health (DA16994, DK28172).

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