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Fig. S1. Ventral brainstem surface markers for the generation of brainstem-spinal cords (BSC) with identified boundaries.

Fig. S2. Brainstem marker nuclei for localizing respiratory structures in newborn rat brainstem-spinal cord preparations.

Fig. S3. Determination of the rostral brainstem-spinal cord boundary for transection levels lacking histological respiratory marker regions.

Fig. S4. Absence of inspiratory rhythm in a brainstem-spinal cord preparation with the rostral boundary at the proposed centre of the preBötC.

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