High-resolution simultaneous voltage and Ca2+ imaging


Corresponding author M. Canepari: INSERM U836 – Grenoble Institute of Neuroscience, Bâtiment Edmond Safra, Chemin Fortune Ferrini, Site santé de la Tronche – BP 170, 38042 Grenoble cedex 9, France.  Email:


Abstract  Combining voltage and Ca2+ imaging allows the correlation of electrical and chemical activity at sub-cellular level. Here we describe a novel apparatus designed to obtain simultaneous voltage and Ca2+ measurements with single-trial resolution from sites as small as a few microns. These measurements can be obtained with negligible optical cross-talk between the two signals and negligible photo-damage of the preparation. The capability of the technique was assessed recording either from individual neurons in brain slices or from networks of cultured neurons. The present achievements open the gate to many novel physiological investigations requiring simultaneous measurement of voltage and Ca2+ signals.