In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem

Authors

  • Daisuke Sugiyama,

    1. Department of Information Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444-8787, Japan
    2. Department of Anesthesiology and Resuscitology, Shinshu University School of Medicine, Matsumoto 390-8621, Japan
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  • Sung Won Hur,

    1. Department of Information Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444-8787, Japan
    2. Department of Physiology and Department of Brain and Cognitive Sciences, Seoul National University College of Medicine, Yeongeon-dong, Jongro-gu, Seoul 110-799, Korea
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  • Anthony E. Pickering,

    1. School of Physiology and Pharmacology, Medical Sciences Building, University of Bristol, Bristol BS8 1TD, UK
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  • Daisuke Kase,

    1. Department of Information Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444-8787, Japan
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  • Sang Jeong Kim,

    1. Department of Physiology and Department of Brain and Cognitive Sciences, Seoul National University College of Medicine, Yeongeon-dong, Jongro-gu, Seoul 110-799, Korea
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  • Mikito Kawamata,

    1. Department of Anesthesiology and Resuscitology, Shinshu University School of Medicine, Matsumoto 390-8621, Japan
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  • Keiji Imoto,

    1. Department of Information Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444-8787, Japan
    2. School of life Science, The Graduate University for Advanced Studies (SOKENDAI), Myodaiji, Okazaki 444-8787, Japan
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  • Hidemasa Furue

    1. Department of Information Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444-8787, Japan
    2. School of life Science, The Graduate University for Advanced Studies (SOKENDAI), Myodaiji, Okazaki 444-8787, Japan
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H. Furue: Department of Information Physiology, National Institute for Physiological Sciences, 5-1 Higashiyama, Myodaiji, Okazaki 444-8787, Japan.  Email: furue@nips.ac.jp

Abstract

Abstract  Locus coeruleus (LC) neurones extend noradrenergic projections throughout the neuroaxis and are involved in homeostatic functions such as pain modulation, arousal and cardio-respiratory control. To address the cellular mechanisms underlying pain modulation we have developed a patch-clamp recording technique from LC neurones in anaesthetized rats. These recordings showed LC discharge in vivo to be driven by both spontaneous membrane potential oscillations and CNQX-sensitive EPSCs opposed by bicuculine-sensitive IPSCs. Hindlimb pinch evoked a biphasic action potential response underpinned by a slow monophasic excitatory current. This approach allows detailed characterisation of the synaptic and integrative mechanisms of LC responses to naturalistic stimulation.

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