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Growth and Differentiation of Astrocytes and Neural Progenitor Cells on Micropatterned Polymer Films

Authors

  • JENNIFER B. RECKNOR,

    1. Department of Chemical Engineering, Iowa State University, Ames, Iowa 50011, USA
    2. The Neuroscience Program, Iowa State University, Ames, Iowa 50011, USA
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  • D S. SAKAGUCHI,

    1. Department of Chemical Engineering, Iowa State University, Ames, Iowa 50011, USA
    2. Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, Iowa 50011, USA
    3. The Neuroscience Program, Iowa State University, Ames, Iowa 50011, USA
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  • S.K. MALLAPRAGADA

    Corresponding author
    1. Department of Chemical Engineering, Iowa State University, Ames, Iowa 50011, USA
    2. The Neuroscience Program, Iowa State University, Ames, Iowa 50011, USA
    • Address for correspondence: S.K. Mallapragada, 3035 Sweeney Hall, Iowa State University, Ames, IA 50011. Voice: + 1-515-294-7407; fax + 1-515-294-2689. suryakm@iastate.edu

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Abstract

Abstract: This paper investigates the influence of micropatterned polymers and chemical modification on neural progenitor cell growth and differentiation in co-culture systems with astrocytes. We sought to develop stategies to facilitate nerve regeneration using a synergistic combination of guidance cues, investigating the cellular mechanisms of nerve repair using adult rat hippocampal progenitor cells (AHPCs). Our studies have shown that this synergistic combination of physical, chemical, and biological cues can lead to oriented growth of astrocytes and progenitor cells, can control and accelerate neurite outgrowth and alignment in vitro, and may influence differentiation of progenitor cells.

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