Proteasomal Oscillation during Mild Heat Shock in Aging Human Skin Fibroblasts

Authors

  • DAVID CHRISTIAN KRAFT,

    1. Laboratory of Cellular Aging, Danish Centre for Molecular Gerontology, Department of Molecular Biology, University of Aarhus, Denmark
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  • CUSTER C. DEOCARIS,

    1. Gene Function Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Japan
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  • SURESH I. S. RATTAN

    1. Laboratory of Cellular Aging, Danish Centre for Molecular Gerontology, Department of Molecular Biology, University of Aarhus, Denmark
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Address for correspondence: Dr. Suresh I.S. Rattan, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK8000 Aarhus−C, Denmark. Voice: +45 8942 5034; fax: +45 8612 3178.
 e-mail: rattan@mb.au.dk

Abstract

Abstract: Augmentation of proteasome machinery is emerging as a significant gerontomodulatory consequence of hormetic stimulation, such as mild heat stress. This study describes the phenomenon we term hormetic proteasomal oscillation, wherein mildly heat-stressed human fibroblasts (41°C, 1 h) display an adaptation response pattern in proteasome activity. Remarkably, such response appears to be diverse in severely heat-stressed or senescent fibroblasts. This proteasomal oscillation, as an innate cellular reaction to heat and aging, however, is independent of 20S proteasome protein levels and nuclear factor-E2-related factor 2 (Nrf2) transactivation.

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