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Oxytocin Receptor Is Differentially Expressed in Mouse Endometrium and Embryo during Blastocyst Implantation

Authors

  • PANAGIOTIS BERETSOS,

    1. First Department of Obstetrics and Gynecology, Division of Reproductive Medicine, Athens University Medical School, Athens, Greece
    2. Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Athens, Greece
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  • DIMITRIS LOUTRADIS,

    1. First Department of Obstetrics and Gynecology, Division of Reproductive Medicine, Athens University Medical School, Athens, Greece
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  • STAUROS KOUSSOULAKOS,

    1. Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Athens, Greece
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  • LOUKAS H. MARGARITIS,

    1. Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Athens, Greece
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  • ERASMIA KIAPEKOU,

    1. First Department of Obstetrics and Gynecology, Division of Reproductive Medicine, Athens University Medical School, Athens, Greece
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  • GEORGE MASTORAKOS,

    1. Endocrine Unit, Second Department of Obstetrics and Gynecology, Aretaieion Hospital, University of Athens, Athens, Greece
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  • IRINI PAPASPIROU,

    1. First Department of Obstetrics and Gynecology, Division of Reproductive Medicine, Athens University Medical School, Athens, Greece
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  • NIKOLAOS MAKRIS,

    1. First Department of Obstetrics and Gynecology, Division of Reproductive Medicine, Athens University Medical School, Athens, Greece
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  • ANTONIS MAKRIGIANNAKIS,

    1. Laboratory of Human Reproduction, Department of Obstetrics and Gynecology, Medical School, University of Crete, Heraklion, Greece
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  • ARIS ANTSAKLIS

    1. First Department of Obstetrics and Gynecology, Division of Reproductive Medicine, Athens University Medical School, Athens, Greece
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Address for correspondence: Panagiotis Beretsos, First Department of Obstetrics and Gynecology, Division of Reproductive Medicine, Athens University Medical School, 50 Achaias St., 115 23, Athens, Greece. Voice: 0030-694-6902173; fax: 0030-210-7470460.
 e-mail: pberet@biol.uoa.gr

Abstract

Abstract: The oxytocin (OT)-oxytocin receptor (OTR) system of the mammalian uterus has mainly been studied in relation to its involvement in the onset of labor. The aim of this study was to elucidate the in vivo expression and localization pattern of OTR in the mouse endometrium and embryo during implantation, as well as OTR mRNA expression in the in vitro developing mouse embryo. The expression of OTR or OT was detected immunohistochemically in uterine tissue sections of 5- to 8-week-old female mice between days 4 and 10 of an established pregnancy. In addition, the expression of OTR mRNA was detected by means of reverse transcription polymerase chain reaction (RT-PCR) in mouse oocytes and embryos up to the blastocyst stage. The mean ratios of normalized expression levels of OTR gene in all samples were also calculated. The recorded increase in OTR mRNA immediately after fertilization could mean a possible role of OT in this process, as OTR mRNA gradually decreased after the four-cell stage of pre-embryonic development. The differential expression of OTR during embryonic apposition and embryonic invasion/placentation in the mouse uterus suggests a potential role of OT in the implantation process of the mouse. It is possible that the interaction of OTR with the hormones included in the ovulation induction regiments utilized today in in vitro fertilization (IVF) could be affecting the receptivity/quality of the implanting endometrium.

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