Abstract: Our aim was to determine the presence of Rickettsia spp. and Bartonella spp. in Ctenocephalides felis and Ctenocephalides canis from La Rioja (Spain). A total of 88 specimens were tested by polymerase chain reaction (PCR) using gltA and ompB genes as targets for Rickettsia spp., and 16S rRNA and ribC genes for Bartonella spp. Rickettsia felis-like (28.4%), Bartonella clarridgeiae (6.8%), and Bartonella henselae (3.4%) were detected in Ctenocephalides spp. Other Bartonella sp. different from B. clarridgeiae and B. henselae could also be present in fleas from La Rioja.