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Highly Specific Novel Method for Isolation and Purification of Lupus Anti-DNA Antibody via Oligo-(dT) Magnetic Beads
Article first published online: 29 AUG 2007
DOI: 10.1196/annals.1422.023
Issue
Annals of the New York Academy of Sciences
Volume 1108, Autoimmunity, Part D: Autoimmune Disease, Annus Mirabilis pages 203–217, June 2007
Additional Information
How to Cite
PAVLOVIC, M., CHEN, R., KATS, A. M., CAVALLO, M. F., SACCOCIO, S., KEATING, P. and HARTMANN, J. X. (2007), Highly Specific Novel Method for Isolation and Purification of Lupus Anti-DNA Antibody via Oligo-(dT) Magnetic Beads. Annals of the New York Academy of Sciences, 1108: 203–217. doi: 10.1196/annals.1422.023
Publication History
- Issue published online: 29 AUG 2007
- Article first published online: 29 AUG 2007
- Abstract
- Article
- References
- Cited By
Keywords:
- systemic lupus erythematosus (SLE);
- anti-ssDNA antibody purification;
- magnetic beads;
- affinity chromatography;
- SDS-PAGE;
- silver staining;
- recovery rate;
- antibody hydrolytic activity
Abstract: A novel method for isolation and purification of anti-ssDNA antibodies from human sera is developed. The process involves: antibody purification based on their affinity for single-stranded sequence of thymidines and removal of remaining components via protein G coated magnetic beads, with high affinity for only IgG subclass. The high degree of purity and molecular weights of healthy versus lupus anti-ssDNA antibodies were confirmed by SDS-PAGE and silver staining. Western blot confirmed IgG isotype. This novel technique allows for diagnostic purposes, structural and functional analysis of anti-DNA antibodies, and studies of their role in autoimmune diseases.