Two-photon Excitation Fluorescence Bioassays

  1. Pekka Hänninen1,
  2. Jori Soukka2,
  3. Juhani T. Soini3

Article first published online: 28 JUN 2008

DOI: 10.1196/annals.1430.040

Issue

Annals of the New York Academy of Sciences

Annals of the New York Academy of Sciences

Additional Information

How to Cite

Hänninen, P., Soukka, J. and Soini, J. T. (2008), Two-photon Excitation Fluorescence Bioassays. Annals of the New York Academy of Sciences, 1130: 320–326. doi: 10.1196/annals.1430.040

Author Information

  1. 1

    Department of Cell Biology and Anatomy, Laboratory of Biophysics, University of Turku, Turku, Finland

  2. 2

    ArcDia, Inc., Turku, Finland

  3. 3

    Turku University of Applied Sciences, Turku, Finland

*Address for correspondence: Pekka Hänninen, Department of Cell Biology and Anatomy, Laboratory of Biophysics, University of Turku, 20014 Turku, Finland.  pekka.hanninen@utu.fi

Publication History

  1. Issue published online: 28 JUN 2008
  2. Article first published online: 28 JUN 2008

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (167K)

Keywords:

  • two-photon excitation;
  • bioassay;
  • in vitro diagnostics

Application of two-photon excitation of fluorescence in microscopy is one of the major discoveries of the “renaissance” of light microscopy that started in the 1980s. The technique derives its advantages from the biologically “smooth” wavelength of the excitation light and the confinement of the excitation. Difficult, and seemingly nontransparent, samples may be imaged with the technique with good resolution. Although the bioresearch has been concentrating mostly on the positive properties of the technique for imaging, the same properties may be applied successfully to nonimaging bioassays. This article focuses on the development path of two-photon excitation–based assay system.

View Full Article (HTML) Get PDF (167K)