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Tumor Necrosis Factor Alpha Inducing Spatial Interactions between Calcium-Sensing Receptor and L-Type Voltage-Dependent Calcium Channel

Authors

  • Jai Parkash

    1. Department of Environmental and Occupational Health, Stempel School of Public Health, Florida International University, Miami, Florida, USA
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Address for correspondence: Jai Parkash, Department of Environmental and Occupational Health, Stempel School of Public Health, Florida International University, 11200 SW 8th Street, Room HLS 594, Miami, FL 33199. Voice: +305-348-7791; fax: +305-348-4901. parkashj@fiu.edu

Abstract

The temporal and spatial regulation of intracellular calcium concentration ([Ca2+]i) is very crucial for maintaining calcium ion homeostasis within cells, and consequently in the regulation of cellular functions such as β cell replication and differentiation, insulin secretion, and apoptosis. Calcium ion regulatory proteins playing major roles in these processes include L-type voltage-dependent calcium channels (L-type VDCCs) and calcium-sensing receptors (CaRs). Tumor necrosis factor alpha (TNF-α), a cytokine, is widely known to activate nuclear factor-kappa B (NF-κB) transcription in β cells. Confocal fluorescence imaging data suggest increased co-localization of CaRs with L-type VDCCs upon treatment of β cells with TNF-α, thereby indicating increased membrane-delimited spatial interactions between these two membrane proteins.

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