Circulating DNA in the Blood of Gastric Cancer Patients

Authors

  • Elena V. Kolesnikova,

    1. Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, 630090 Russia
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  • Svetlana N. Tamkovich,

    1. Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, 630090 Russia
    2. Novosibirsk State University, Novosibirsk, 630090 Russia
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  • Olga E. Bryzgunova,

    1. Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, 630090 Russia
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  • Petr I. Shelestyuk,

    1. Novosibirsk Oncological Dispensar, Novosibirsk, 630108 Russia
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  • Valentina I. Permyakova,

    1. Central Clinical Hospital, Siberian Division of the Russian Academy of Sciences, Novosibirsk, 630090 Russia
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  • Valentin V. Vlassov,

    1. Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, 630090 Russia
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  • Aleksandr S. Tuzikov,

    1. Cancer Research Institute, Siberian Division of the Russian Academy of Medical Sciences, Tomsk, 634009 Russia
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  • Pavel P. Laktionov,

    1. Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, 630090 Russia
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  • Elena Y. Rykova

    1. Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, 630090 Russia
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Address for correspondence: Elena Y. Rykova, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, pr. Lavrentiev 8, Novosibirsk, 630090 Russia. Voice: +7-383-330-46-54; fax: +7-383-333-36-77. rykova@niboch.nsc.ru

Abstract

The concentration of cell-free DNA and promoter methylation status of the MGMT, p15, and hMLH1 genes were analyzed by a fluorescence-based assay and methylation-specific PCR (MSP) in the blood of gastric cancer patients (n= 20) and healthy subjects (n= 22). Gastric cancer patients were characterized by an increased concentration of circulating DNA in the plasma; the amount of cell-surface-bound DNA was not decreased compared with controls and amounted to 80 ± 15% of the total circulating DNA. MSP analysis of three genes in the cell-surface-bound DNA permits the detection of gastric cancer patients with a sensitivity of 75% and a specificity of 54%. Thus, the cell-surface-bound DNA is a convenient source of DNA for MSP analysis of cancer-specific markers. The data on the presence of methylated DNA in plasma combined with the analysis of other cancer-related changes in DNA can significantly contribute to cancer diagnostics.

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