2042-7158/asset/olbannerleft.jpg?v=1&s=690c0bb87ea13175753e0575c06a052fadf3d184)
2042-7158/asset/olbannerright.jpg?v=1&s=6190cbe6e02b0c4682063fe6ea1b962f97a9d725)
Estimation of Individual Sennosides in Plant Materials and Marketed Formulations by an HPTLC Method
Article first published online: 18 FEB 2010
DOI: 10.1211/0022357001774066
2000 Royal Pharmaceutical Society of Great Britain
2042-7158/asset/cover.gif?v=1&s=f696abb7b9dc2d7091065df4c2e6dd2b3b4df93a "Journal of Pharmacy and Pharmacology")
Additional Information
How to Cite
SHAH, S. A., RAVISHANKARA, M. N., NIRMAL, A., SHISHOO, C. J., RATHOD, I. S. and SUHAGIA, B. N. (2000), Estimation of Individual Sennosides in Plant Materials and Marketed Formulations by an HPTLC Method. Journal of Pharmacy and Pharmacology, 52: 445–449. doi: 10.1211/0022357001774066
Publication History
- Issue published online: 18 FEB 2010
- Article first published online: 18 FEB 2010
- Received September 28, 1999, Accepted December 20, 1999
- Abstract
- References
- Cited By
Abstract
Senna is a well-known drug, used in the Ayurvedic and Allopathic systems of medicine, and is a treatment for constipation. The purgative action of senna and its formulations is due to the presence of sennosides A and B. An HPTLC method has been developed for the determination of individual sennosides (A, B, C, D) without any derivatization in marketed formulations (three tablet formulations, two granule formulations and one liquid formulation) and plant materials (senna leaf and pod).
The methanolic solution of a sample was applied on a pre-coated silica gel G60 F254 TLC plate (E. Merck.) and was developed using n-propanol: ethyl acetate: water: glacial acetic acid (3:3:2:0.1 v/v) as the mobile phase. The relative band speeds (Rf values) obtained were 0.35, 0.25, 0.61, 0.46 for sennosides A, B, C and D, respectively. The densitometric response was monitored at 366 nm. Calibration curves were found to be linear in the concentration ranges 193–1356, 402–2817, 71–497 and 132–927 ng per spot for sennosides A, B, C, and D, respectively. The correlation coefficients were found to be 0.9978, 0.9987, 0.9939 and 0.9983 respectively for sennosides A, B, C and D. The result obtained with the HPTLC method for total sennoside content was compared with the results using the pharmacopoeial methods (spectrophotometric (British Pharmacopoeia) and spectrofluorimetric (United States Pharmacopeia) using the ‘F’ test). The results revealed no significant difference in the three different methods for estimation of total sennoside.
The proposed HPTLC method was found to be simple, specific, precise, accurate and rapid. It can be used for routine quality control of sennosides or senna-containing formulations for individual sennosides.