Identification of potential anti-photoageing algal compounds using an in-vitro model of photoageing

Authors

  • F. Debacq-Chainiaux,

    1. Laboratory of Biochemistry and Cellular Biology, Department of Biology, University of Namur (FUNDP), Belgium. F. Debacq-Chainiaux, C. Borlon, J. Remacle, O. Toussaint
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  • C. Borlon,

    1. Laboratory of Biochemistry and Cellular Biology, Department of Biology, University of Namur (FUNDP), Belgium. F. Debacq-Chainiaux, C. Borlon, J. Remacle, O. Toussaint
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  • B. De Hertogh,

    1. Research Unit in Molecular Biology (URBM), Department of Biology, University of Namur (FUNDP), Belgium. B. De Hertogh
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  • J. Remacle,

    1. Laboratory of Biochemistry and Cellular Biology, Department of Biology, University of Namur (FUNDP), Belgium. F. Debacq-Chainiaux, C. Borlon, J. Remacle, O. Toussaint
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  • P.-Y. Morvan,

    1. Codif International, St-Malo, France. P.-Y. Morvan, R. Vallée
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  • R. Vallée,

    1. Codif International, St-Malo, France. P.-Y. Morvan, R. Vallée
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  • O. Toussaint

    Corresponding author
    1. Laboratory of Biochemistry and Cellular Biology, Department of Biology, University of Namur (FUNDP), Belgium. F. Debacq-Chainiaux, C. Borlon, J. Remacle, O. Toussaint
    2. StratiCELL Screening Technologies S.A., Namur, Belgium. O. Toussaint
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Rue de Bruxelles, 61 B-5000 Namur, Belgium. E-mail: olivier.toussaint@fundp.ac.be

Abstract

Stress-induced premature senescence (SIPS) has been proposed as an in-vitro model for testing the long-term effects of stressful events and to find molecules/natural extracts that protect against such stress. Premature senescence of human skin diploid fibroblasts (HDFs) can be induced by repeated subcytotoxic exposure to UVB, with the appearance of so-called biomarkers of senescence such as growth arrest, senescence-associated β-galactosidase activity, senescence-associated gene over-expression and the common 4977-bp mitochondrial DNA deletion. This model of UVB-induced premature senescence has been acknowledged as a robust in-vitro model in photoageing research. In this study, the potential anti-photoageing effects of a series of algal extracts were tested. The appearance of the biomarkers of UVB-induced premature senescence of HDFs was studied with or without algal extracts. One algal extract was shown to be particularly protective against UVB-induced SIPS. The results obtained here reinforce the notion that UVB-induced premature senescence of HDFs can be used to screen potential anti-photoageing compounds.

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