Silymarin inhibits melanin synthesis in melanocyte cells
Article first published online: 8 JAN 2010
2009 Royal Pharmaceutical Society of Great Britain
Journal of Pharmacy and Pharmacology
Volume 61, Issue 5, pages 663–667, May 2009
How to Cite
Choo, S.-J., Ryoo, I.-J., Kim, Y.-H., Xu, G.-H., Kim, W.-G., Kim, K.-H., Moon, S.-J., Son, E.-D., Bae, K. and Yoo, I.-D. (2009), Silymarin inhibits melanin synthesis in melanocyte cells. Journal of Pharmacy and Pharmacology, 61: 663–667. doi: 10.1211/jpp.61.05.0016
- Issue published online: 8 JAN 2010
- Article first published online: 8 JAN 2010
- Received December 04, 2008; Accepted January 26, 2009
- tyrosinase activity
Objectives The aim was to search for inhibitors of melanogenesis from natural resources.
Methods The inhibitory effect of silymarin on melanogenesis in a spontaneously immortalized mouse melanocyte cell line, Mel-Ab, was studied.
Key findings Silymarin significantly prevented melanin production in a dose-dependent manner with an IC50 value (concentration producing 50% maximal inhibition) of 28.2 μg/ml, without effects on cell viability. Also, silymarin inhibited l-DOPA oxidation activity of tyrosinase, the rate-limiting melanogenic enzyme, in cell based-systems but it did not directly affect cell-free tyrosinase activity. Furthermore, Western blot analysis indicated that silymarin decreased the expression of tyrosinase protein.
Conclusions This study suggests that the depigmenting effect of silymarin might be attributable to inhibition of tyrosinase expression and that silymarin may be useful as a natural skin-lightening agent.