ICTA PM, Fontaine-lès-Dijon, France.
Electroosmotic transport of mannitol across human nail during constant current iontophoresis
Version of Record online: 10 JUN 2010
© 2010 The Authors Journal compilation © 2010 Royal Pharmaceutical Society of Great Britain
Journal of Pharmacy and Pharmacology
Special Issue: Recent advances in transdermal drug delivery
Volume 62, Issue 6, pages 721–729, June 2010
How to Cite
Dutet, J. and Delgado-Charro, M. B. (2010), Electroosmotic transport of mannitol across human nail during constant current iontophoresis. Journal of Pharmacy and Pharmacology, 62: 721–729. doi: 10.1211/jpp.62.06.0008
- Issue online: 10 JUN 2010
- Version of Record online: 10 JUN 2010
- Received October 30, 2009Accepted February 09, 2010
Objectives This work aimed to elucidate the role of electroosmosis during trans-nail iontophoresis.
Methods Passive and iontophoretic experiments were performed after short hydration (10–15 min) of human nail tips. The electroosmotic fluxes of mannitol were determined during anodal and cathodal iontophoresis and at different pH values. Passive controls were also carried out. Four sets of experiments were performed: (a) three anodal delivery experiments using different nails, at pH 4.0, 5.0 and 7.4, (b) one anodal delivery experiment that kept the same nails across two pH stages, (c) one experiment, comprising an anodal delivery stage (pH 4 and 7.4) followed by a cathodal delivery stage (pH 7.4 and 4), which kept the same nails across the different polarities and pH stages, and (d) a passive experiment keeping the same nails across different pH values (4 and 7.4).
Results The fluxes of mannitol measured were very variable and little difference between passive and electroosmotic transport was observed. Cathodal and anodal fluxes were not always significantly different. Experiments which minimised internail variability suggested that the nails were negatively charged at physiological pH, and that this negative charge was lost at pH 4.
Conclusions These results suggest a modest and highly variable contribution of electroosmosis to the iontophoretic transungual flux.