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Objectives. This research aimed to develop and validate a measure of integrated regulation based on self-determination theory (SDT) in an exercise context.

Design. Cross-sectional and prospective surveys were employed.

Methods. The measure was developed from first principles from an initial item pool and items were selected using expert evaluators. The validity of the final item pool was tested across a high-active sample and a lower active sample (N= 488) using single- and multi-sample confirmatory factor analyses (CFAs).

Results. Analyses supported the factorial, nomological, discriminant, and predictive validity of the scale.

Conclusions. The validity of the integrated regulation measure was supported. Present analyses provide evidence that the scale is a valid and reliable tool that may be used to evaluate the effectiveness of autonomy-supportive interventions in health-related behavioural contexts.