• Please log in or register to access this feature.

Research-Article

You have full text access to this OnlineOpen article

Upregulation of Osteopontin by Osteocytes Deprived of Mechanical Loading or Oxygen†

  1. Ted S Gross PhD*,
  2. Katy A King,
  3. Natalia A Rabaia,
  4. Pranali Pathare,
  5. Sundar Srinivasan

Article first published online: 11 OCT 2004

DOI: 10.1359/JBMR.041004

Issue

Journal of Bone and Mineral Research

Journal of Bone and Mineral Research

Volume 20, Issue 2, pages 250–256, February 2005

Additional Information

How to Cite

Gross, T. S., King, K. A., Rabaia, N. A., Pathare, P. and Srinivasan, S. (2005), Upregulation of Osteopontin by Osteocytes Deprived of Mechanical Loading or Oxygen. J Bone Miner Res, 20: 250–256. doi: 10.1359/JBMR.041004

Author Information

  1. Orthopaedic Science Laboratories, Department of Orthopaedics and Sports Medicine, University of Washington, Seattle, Washington, USA

*Department of Orthopaedics and Sports Medicine, 325 Ninth Avenue, Box 359798, Seattle, WA 98104-2499, USA

  1. †

    The authors have no conflict of interest.

Publication History

  1. Issue published online: 4 DEC 2009
  2. Article first published online: 11 OCT 2004
  3. Manuscript Revised: 30 AUG 2004
  4. Manuscript Accepted: 30 AUG 2004
  5. Manuscript Received: 3 FEB 2004

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (225K)

REFERENCES

  • 1
    Leblanc AD, Schneider VS, Evans HJ, Engelbretson DA, Krebs JM 1990 Bone mineral loss and recovery after 17 weeks of bed rest. J Bone Miner Res 5: 843–850.
  • 2
    Dehority W, Halloran BP, Bikle DD, Curren T, Kostenuik PJ, Wronski TJ, Shen Y, Rabkin B, Bouraoui A, Morey-Holton E 1999 Bone and hormonal changes induced by skeletal unloading in the mature male rat. Am J Physiol 276: E62–E69.
  • 3
    Gross TS, Rubin CT 1995 Uniformity of resorptive bone loss induced by disuse. J Orthop Res 13: 708–714.
  • 4
    Troen BR 2003 Molecular mechanisms underlying osteoclast formation and activation. Exp Gerontol 38: 605–614.
  • 5
    Ishijima M, Tsuji K, Rittling SR, Yamashita T, Kurosawa H, Denhardt DT, Nifuji A, Noda M 2002 Resistance to unloading-induced three-dimensional bone loss in osteopontin-deficient mice. J Bone Miner Res 17: 661–667.
  • 6
    Yoshitake H, Rittling SR, Denhardt DT, Noda M 1999 Osteopontin-deficient mice are resistant to ovariectomy-induced bone resorption. Proc Natl Acad Sci USA 96: 8156–8160.
  • 7
    Ihara H, Denhardt DT, Furuya K, Yamashita T, Muguruma Y, Tsuji K, Hruska KA, Higashio K, Enomoto S, Nifuji A, Rittling SR, Noda M 2001 Parathyroid hormone-induced bone resorption does not occur in the absence of osteopontin. J Biol Chem 276: 13065–13071.
  • 8
    Weber GF, Cantor H 1996 The immunology of Eta-1/osteopontin. Cytokine Growth Factor Rev 7: 241–248.
  • 9
    Denhardt DT, Noda M 1998 Osteopontin expression and function: Role in bone remodeling. J Cell Biochem Suppl 30–31: 92–102.
  • 10
    Suzuki K, Zhu B, Rittling SR, Denhardt DT, Goldberg HA, McCulloch CA, Sodek J 2002 Colocalization of intracellular osteopontin with CD44 is associated with migration, cell fusion, and resorption in osteoclasts. J Bone Miner Res 17: 1486–1497.
  • 11
    Ajubi NE, Klein-Nulend J, Nijweide PJ, Vrijheid-Lammers T, Alblas MJ, Burger EH 1996 Pulsating fluid flow increases prostaglandin production by cultured chicken osteocytes-a cytoskeleton-dependent process. Biochem Biophys Res Commun 225: 62–68.
  • 12
    Sun YQ, McLeod KJ, Rubin CT 1995 Mechanically induced periosteal bone formation is paralleled by the upregulation of collagen type one mRNA in osteocytes as measured by in situ reverse transcript-polymerase chain reaction. Calcif Tissue Int 57: 456–462.
  • 13
    Zhao S, Zhang YK, Harris S, Ahuja SS, Bonewald LF 2002 MLO-Y4 osteocyte-like cells support osteoclast formation and activation. J Bone Miner Res 17: 2068–2079.
  • 14
    Rubin C, Sun YQ, Hadjiargyrou M, McLeod K 1999 Increased expression of matrix metalloproteinase-1 in osteocytes precedes bone resorption as stimulated by disuse: Evidence for autoregulation of the cell's mechanical environment? J Orthop Res 17: 354–361.
  • 15
    Dodd JS, Raleigh JA, Gross TS 1999 Osteocyte hypoxia: A novel mechanotransduction pathway. Am J Physiol 277: C589–C602.
  • 16
    Gross TS, Akeno N, Clemens TL, Komarova S, Srinivasan S, Weimer DA, Mayorov S 2001 Selected Contribution: Osteocytes upregulate HIF-1alpha in response to acute disuse and oxygen deprivation. J Appl Physiol 90: 2514–2519.
  • 17
    Hampel DJ, Sansome C, Romanov VI, Kowalski AJ, Denhardt DT, Goligorsky MS 2003 Osteopontin traffic in hypoxic renal epithelial cells. Nephron Exp Nephrol 94: e66–e76.
  • 18
    Sodhi CP, Phadke SA, Batlle D, Sahai A 2001 Hypoxia stimulates osteopontin expression and proliferation of cultured vascular smooth muscle cells: Potentiation by high glucose. Diabetes 50: 1482–1490.
  • 19
    Lee MY, Shin SL, Choi YS, Kim EJ, Cha JH, Chun MH, Lee SB, Kim SY 1999 Transient upregulation of osteopontin mRNA in hippocampus and striatum following global forebrain ischemia in rats. Neurosci Lett 271: 81–84.
  • 20
    Rubin CT, Lanyon LE 1984 Regulation of bone formation by applied dynamic loads. J Bone Joint Surg Am 66: 397–402.
  • 21
    Rittling SR, Feng F 1998 Detection of mouse osteopontin by western blotting. Biochem Biophys Res Commun 250: 287–292.
  • 22
    Gross TS, Damji AA, Judex S, Bray RC, Zernicke RF 1999 Bone hyperemia precedes disuse-induced intracortical bone resorption. J Appl Physiol 86: 230–235.
  • 23
    Srinivasan S, Weimer DA, Agans SC, Bain SD, Gross TS 2002 Low-magnitude mechanical loading becomes osteogenic when rest is inserted between each load cycle. J Bone Miner Res 17: 1613–1620.
  • 24
    Denhardt DT, Giachelli CM, Rittling SR 2001 Role of osteopontin in cellular signaling and toxicant injury. Annu Rev Pharmacol Toxicol 41: 723–749.
  • 25
    Boskey AL, Spevak L, Paschalis E, Doty SB, McKee MD 2002 Osteopontin deficiency increases mineral content and mineral crystallinity in mouse bone. Calcif Tissue Int 71: 145–154.
  • 26
    Zhu JX, Sasano Y, Takahashi I, Mizoguchi I, Kagayama M 2001 Temporal and spatial gene expression of major bone extracellular matrix molecules during embryonic mandibular osteogenesis in rats. Histochem J 33: 25–35.
  • 27
    Yamazaki M, Nakajima F, Ogasawara A, Moriya H, Majeska RJ, Einhorn TA 1999 Spatial and temporal distribution of CD44 and osteopontin in fracture callus. J Bone Joint Surg Br 81: 508–515.
  • 28
    Terai K, Takano-Yamamoto T, Ohba Y, Hiura K, Sugimoto M, Sato M, Kawahata H, Inaguma N, Kitamura Y, Nomura S 1999 Role of osteopontin in bone remodeling caused by mechanical stress. J Bone Miner Res 14: 839–849.
  • 29
    Ikeda T, Yamaguchi A, Yokose S, Nagai Y, Yamato H, Nakamura T, Tsurukami H, Tanizawa T, Yoshiki S 1996 Changes in biological activity of bone cells in ovariectomized rats revealed by in situ hybridization. J Bone Miner Res 11: 780–788.
  • 30
    Chen J, Singh K, Mukherjee BB, Sodek J 1993 Developmental expression of osteopontin (OPN) mRNA in rat tissues: Evidence for a role for OPN in bone formation and resorption. Matrix 13: 113–123.
  • 31
    You J, Reilly GC, Zhen X, Yellowley CE, Chen Q, Donahue HJ, Jacobs CR 2001 Osteopontin gene regulation by oscillatory fluid flow via intracellular calcium mobilization and activation of mitogen-activated protein kinase in MC3T3-E1 osteoblasts. J Biol Chem 276: 13365–13371.
  • 32
    Miles RR, Turner CH, Santerre R, Tu Y, McClelland P, Argot J, DeHoff BS, Mundy CW, Rosteck PR Jr, Bidwell J, Sluka JP, Hock J, Onyia JE 1998 Analysis of differential gene expression in rat tibia after an osteogenic stimulus in vivo: Mechanical loading regulates osteopontin and myeloperoxidase. J Cell Biochem 68: 355–365.
  • 33
    King KA, Rabia NA, Zhang Z, Srinivasan S, Gross TS 2003 Spatial mediation of intracortical resorption. J Bone Miner Res 18: S1; S73.
  • 34
    Razzouk S, Brunn JC, Qin C, Tye CE, Goldberg HA, Butler WT 2002 Osteopontin posttranslational modifications, possibly phosphorylation, are required for in vitro bone resorption but not osteoclast adhesion. Bone 30: 40–47.
  • 35
    Tami AE, Schaffler MB, Knothe Tate ML 2003 Probing the tissue to subcellular level structure underlying bone's molecular sieving function. Biorheology 40: 577–590.
  • 36
    Ohno S, Nishizuka Y 2002 Protein kinase C isotypes and their specific functions: Prologue. J Biochem (Tokyo) 132: 509–511.
  • 37
    Conte M, Soper B, Chang Q, Tepperman B 2004 The role of PKCdelta and PKCepsilon in the neonatal rat colon in response to hypoxia challenge. Pediatr Res 55: 27–33.
  • 38
    Toma CD, Ashkar S, Gray ML, Schaffer JL, Gerstenfeld LC 1997 Signal transduction of mechanical stimuli is dependent on microfilament integrity: Identification of osteopontin as a mechanically induced gene in osteoblasts. J Bone Miner Res 12: 1626–1636.
  • 39
    Chang PL, Tucker MA, Hicks PH, Prince CW 2002 Novel protein kinase C isoforms and mitogen-activated kinase kinase mediate phorbol ester-induced osteopontin expression. Int J Biochem Cell Biol 34: 1142–1151.
  • 40
    Kortmansky J, Schwartz GK 2003 Bryostatin-1: A novel PKC inhibitor in clinical development. Cancer Invest 21: 924–936.
  • 41
    Advani R, Peethambaram P, Lum BL, Fisher GA, Hartmann L, Long HJ, Halsey J, Holmlund JT, Dorr A, Sikic BI 2004 A Phase II trial of aprinocarsen, an antisense oligonucleotide inhibitor of protein kinase C alpha, administered as a 21-day infusion to patients with advanced ovarian carcinoma. Cancer 100: 321–326.
  • 42
    Bain SD, Rubin CT 1990 Temporal nature of osteoclast resorption in response to disuse. J Bone Miner Res 5: S2; S217.
  • 43
    Martin RB, Pickett JC, Zinaich S 1980 Studies of skeletal remodeling in aging men. Clin Orthop 149: 268–282.
  • 44
    Young DR, Niklowitz WJ, Steele CR 1983 Tibial changes in experimental disuse osteoporosis in the monkey. Calcif Tissue Int 35: 304–308.
  • 45
    Arnett TR, Gibbons DC, Utting JC, Orriss IR, Hoebertz A, Rosendaal M, Meghji S 2003 Hypoxia is a major stimulator of osteoclast formation and bone resorption. J Cell Physiol 196: 2–8.
  • 46
    Rubin J, Fan X, Biskobing DM, Taylor WR, Rubin CT 1999 Osteoclastogenesis is repressed by mechanical strain in an in vitro model. J Orthop Res 17: 639–645.
  • 47
    Khan SA, Lopez-Chua CA, Zhang J, Fisher LW, Sorensen ES, Denhardt DT 2002 Soluble osteopontin inhibits apoptosis of adherent endothelial cells deprived of growth factors. J Cell Biochem 85: 728–736.
View Full Article (HTML) Get PDF (225K)