The authors have no conflict of interest.
Mice Lacking the Integrin β5 Subunit Have Accelerated Osteoclast Maturation and Increased Activity in the Estrogen-Deficient State†
Article first published online: 25 OCT 2004
Copyright © 2005 ASBMR
Journal of Bone and Mineral Research
Volume 20, Issue 1, pages 58–66, January 2005
How to Cite
Lane, N. E., Yao, W., Nakamura, M. C., Humphrey, M. B., Kimmel, D., Huang, X., Sheppard, D., Ross, F. P. and Teitelbaum, S. L. (2005), Mice Lacking the Integrin β5 Subunit Have Accelerated Osteoclast Maturation and Increased Activity in the Estrogen-Deficient State. J Bone Miner Res, 20: 58–66. doi: 10.1359/JBMR.041017
- Issue published online: 4 DEC 2009
- Article first published online: 25 OCT 2004
- Manuscript Accepted: 11 AUG 2004
- Manuscript Revised: 6 AUG 2004
- Manuscript Received: 14 JUN 2004
- estrogen deficiency
Integrin αvβ5 is expressed on osteoclast precursors and is capable of recognizing the same amino acid motif as αvβ3. Three-month-old β5−/− female OVX mice had increased osteoclastogenesis ex vivo, and μCT assessment of trabecular bone volume was 53% lower than WT-OVX animals. These preliminary data suggest αvβ5 integrin's presence on osteoclast precursors may inhibit of osteoclast formation.
Introduction: Osteoclasts are unique resorptive skeletal cells, capable of degrading bone on contact to the juxtaposed matrix. Integrin αvβ5 is expressed on osteoclast precursors, structurally similar to αvβ3, and capable of recognizing the same amino acid motif. Given the structural relationship and reciprocal regulation of αvβ3 and αvβ5, the purpose of this study was to evaluate how αvβ5 might contribute to osteoclast maturation and activity.
Materials and Methods: Three-month-old wildtype (WT) and β5−/− female mice had ovariectomy (OVX) or sham operations. The osteoclastogenic capacity of marrow-derived precursors, the kinetic, the circulating, and structural parameters of bone remodeling, was determined after 6 weeks of paired feeding.
Results and Conclusions: OVX increased osteoclastogenesis ex vivo and in vivo. Osteoclast formation and prolonged pre-osteoclast survival were substantially enhanced in cultures containing β5−/− cells whether obtained from sham-operated or OVX mice. Expression of cathepsin K, β3 integrin subunit, and calcitonin receptor were accelerated in cultured β5−/−osteoclasts. β5−/− osteoclasts from OVX animals showed a 3-fold enhancement of net resorptive activity, with quantitative μCT showing trabecular bone volume loss after OVX 53% greater in β5−/− OVX compared with similarly treated WT OVX mice (p < 0.05). α5β3 seems to be an inhibitor of osteoclast formation, in contrast to αvβ3. In addition, loss of αvβ5 seems to accelerate osteoclast formation in the OVX model. Further examination of αvβ5 signaling pathways may enhance our understanding of the activation of bone resorption.