Dr Marín is an employee of Eli Lilly, a company that investigates and commercializes bone active drugs. All other authors state that they have no conflicts of interest.
Relationship Between Bone Quantitative Ultrasound and Fractures: A Meta-Analysis†
Article first published online: 15 MAY 2006
Copyright © 2006 ASBMR
Journal of Bone and Mineral Research
Volume 21, Issue 7, pages 1126–1135, July 2006
How to Cite
Marín, F., González-Macías, J., Díez-Pérez, A., Palma, S. and Delgado-Rodríguez, M. (2006), Relationship Between Bone Quantitative Ultrasound and Fractures: A Meta-Analysis. J Bone Miner Res, 21: 1126–1135. doi: 10.1359/jbmr.060417
- Issue published online: 4 DEC 2009
- Article first published online: 15 MAY 2006
- Manuscript Accepted: 28 APR 2006
- Manuscript Revised: 20 APR 2006
- Manuscript Received: 15 FEB 2006
- bone ultrasound;
- bone densitometry;
The relationship between bone QUS and fracture risk was estimated in a systematic review of data from 14 prospective studies of 47,300 individuals and 2350 incident fractures. In older women, low QUS values were associated with overall fracture risk, low-trauma fractures, and with hip, forearm, and humerus fractures separately.
Introduction: Bone quantitative ultrasound (QUS) has emerged as a promising technique to evaluate bone status. The aim of this study was to determine the association between measurements of QUS with the risk of fracture.
Materials and Methods: A meta-analysis of prospective cohort studies published between 1985 and June 2005 with a baseline measurement of QUS and subsequent follow-up for fractures was carried out. Fourteen separate study populations, consisting of about 47,300 individuals (85.4% women), with about 124,000 person-years of observation and over 2350 fractures, including 653 hip, 529 forearm, and 386 humeral fractures, were analyzed. The main outcome measure was the estimated relative risk of fracture for a decrease in bone QUS parameters of 1 SD below sex- and age-adjusted mean in women.
Results: Eleven studies evaluated QUS at the heel, with patella and phalanx (two studies each) and distal radius (one study) being scarcely used. There was not significant heterogeneity among the studies included in the review. Relative risk estimates (95% CI) for overall fractures were 1.55 (1.35–1.78) for each SD decrease in broadband ultrasound attenuation (BUA), 1.63 (1.37–1.93) for speed of sound (SOS), and 1.74 (1.39–2.17) for QUS index/stiffness index (QUI/SI). Risk estimates were similar or slightly higher for hip fractures and low-energy trauma fractures. Humeral and forearm/wrist fractures were also related with lower QUS values.
Conclusions: Measurements of bone QUS are significantly associated with nonspinal fracture risk in older women in a similar degree to DXA. QUS may be a valid alternative to evaluate fracture risk in situations where DXA is not accessible.
The identification of individuals at high risk of osteoporotic fractures, particularly among the elderly, is of pivotal importance to implement effective preventive measures. Fragility fractures occur because a combination of low bone mass and deterioration in the quality properties of the bone, including macro- and microarchitectural changes in the trabecular and cortical bone, and an age-related increase in the risk of falls. Therefore, the current approach to the assessment of fracture risk is based on the evaluation of several risk factors, including age, the existence of previous fractures, a family history of fractures, and several clinical factors related with the bone status and propensity to fall.(1–3) However, the measurement of BMD by DXA is still considered one of the most important predictors of fracture risk in both sexes,(4) probably because it is a measurable factor, and it is one that can be influenced by therapeutic manipulations. However, axial DXA equipment are expensive, nonportable, involve radiation exposure, and are usually restricted to tertiary care hospitals or specialized clinics because dedicated trained personnel is required. Therefore, in some geographical areas, there are clear limitations in the accessibility to this technique.(5) Thus, the introduction of new alternative methods to evaluate the status of the bone would be of interest.
Quantitative bone ultrasound (QUS) is emerging as a low-cost screening technique that is able to identify women at risk for the osteoporosis and that may be used by general practitioners in primary care and in ambulatory settings.(6) QUS parameters permit analysis of some physical properties of bone tissue, which, in turn, are important determinants of bone stiffness, load failure, and fracture risk by providing additional information to bone mass alone.(7) In fact, a number of cross-sectional studies have shown that the values of the parameters measured with QUS, principally at the calcaneus, are lower in women with a history of osteoporotic fracture, regardless of the BMD values determined by DXA.(8–15) A recent review has also concluded that broadband ultrasound attenuation (BUA) at the heel is an independent risk indicator for hip fractures, but the information was limited to two cohorts.(4) Whereas QUS is increasingly incorporated to clinical practice, no firm conclusions have yet been drawn regarding its potential use in fracture risk assessment. For this reason, the present systematic review of the literature for all prospective studies aims to analyze the association of QUS measurements with the risk of fracture.
MATERIALS AND METHODS
Search strategy and study selection
We identified literature and primary studies for this report by searching computer databases of the medical literature (Medline and EMBASE) from 1985 to June 2005. We based our search on the following keywords: “bone and bones,” “bone density,” “quantitative ultrasound,” and “fractures” combined with the different measurement sites in humans. No language limitations were imposed. We identified 493 articles in this way. A hand search in these references was performed according to the prespecified eligibility criteria for inclusion in the present meta-analysis: (1) prospective studies with a baseline measurement with a QUS technique reported in absolute units, (2) fractures have to be the main outcome and must have occurred after bone QUS measurement had been taken, and (3) studies gave a relative risk (RR) estimate (or to allow its estimation) for fractures. With these criteria, 14 studies were identified and included (Fig. 1). Two prospective studies were not used in this review. The study by Porter et al.(16) did not include any RR estimate. The NORA Study reported only T score values of one nonspecified QUS parameter, and absolute values of bone ultrasound parameters were not included.(17) In one instance (EPIDOS Study), there was more than one published report of the same study population at different time-points and fracture locations.(18–21) In this case, we used the most updated published data.
Data were independently extracted by two investigators (FM and MDR) and compared. Methodological quality of the studies was assessed according to STROBE (Strengthening the Reporting of Observational studies in Epidemiology) statement.(22) The following information was summarized: country where the study was performed, study period, sex, age, type of population (general population, ambulatory, or hospital-based), length of follow-up, anatomical measurement site, QUS equipment, QUS parameters with SDs in the study populations, incident fracture sites, fracture rates, type of incident fracture (low energy or any type), RR or related measures (hazard ratio, OR) and its 95% CI, model used to yield RRs, and, if relevant, variables adjusted for in multivariate analyses. Fracture assessment methods were reviewed. Information on all fractures was used regardless of type of trauma. In addition, fractures associated with low trauma (fragility fractures) were analyzed in those series where this information was included. Finally, hip, forearm/wrist, and humerus fractures were considered separately.
Outcome variables and statistical analysis
We chose RR for fracture associated with a decrease in QUS parameters of 1 SD adjusted by age, because it is independent of QUS devices and population characteristics. RR estimates were pooled using as weight their inverse of variance under a random effects model.(23) This model was chosen as simulations have shown that it works better than the fixed effects model when the number of primary studies is <20.(24,25) Heterogeneity was assessed in all estimates applying the Q statistic and using a cut-off level of p = 0.1 to establish its presence.(23) The degree of inconsistency was assessed estimating I2, which describes the percentage of total variation across studies that is caused by heterogeneity rather than to chance.(26,27) All the analyses were stratified by the type of parameter measured by QUS (bone ultrasound attenuation, BUA; speed of sound, SOS; quantitative ultrasound index/stiffness index, QUI/SI). The presence of publication bias was ascertained by a Christmas tree, representing the natural logarithm of RR (x-axis) against its precision [1/SE(ln RR)] in the y-axis, and by the procedures of Egger et al.(28) and Macaskill et al.(29) that were applied for the QUS parameters, and in fragility and all fractures types (all sites combined). The Stata 8-SE package (College Station, TX, USA) was used for statistical analyses.
Description of the study population
Table 1 gives details of the 14 study cohorts, consisting of ∼47,300 individuals, 124,100 person-years of observation, and ∼2350 fractures, including 653 hip, 528 forearm/wrist, and 386 humerus fractures, that were suitable for inclusion in our analysis.(19–21,30–43) Only 71 incident vertebral fractures or deformities were collected. All fractures were verified by health care providers except for one study(32) where fractures were collected by patient self-report.
The studies were carried out in six European Union member States (n = 10), the United States (n = 3), and Switzerland (n = 1; Table 1). In four studies, the mean age at study baseline was <60 years (33.3% of the total population included in the meta-analysis), whereas the majority of studies (57%) assessed populations ⩾70 years of age (59.7% of the population included in the meta-analysis). Only three studies included a male population with a total of ∼6670 individuals (14.1% of the total population included in the meta-analysis); however, most of them (96.7%) were included in a single study(42) that was the only one that provides stratified results by sex; therefore, a separate meta-analysis of the male population was not feasible. Moreover, because it has been shown than average BUA and SOS in men is higher than in women,(44) we focused the analysis in women only. Two studies that provided results pooling both sexes(35,36) were excluded to keep the grouping more homogeneous. The average length of follow-up of the different primary studies ranged from 1.0 to 5.5 years. Sample size was also variable: there are five studies (35.7%) with >5000 people, whereas seven (50%) have <1000 participants. The most frequent measurement site was the calcaneus (11 studies: 78.6%), whereas the patella and the phalanx were used in only two studies each,(30,32,37,40) and the distal radius in a single, small study(37) (Table 1), which precluded a meta-analysis of the results of noncalcaneus measurement sites.
Heterogeneity and publication bias
We did not observe any heterogeneity for the fractures outcome (either all fractures or low-trauma fractures) for any of the three QUS parameters analyzed (BUA, SOS, and QUI/SI; p > 0.25). The degree of inconsistency was low: I2 values were usually <20% and all of the 95% CIs included the null value. A Christmas tree for the association between BUA and fracture risk (any site) did not reveal any relevant asymmetry (Fig. 2). Similarly, regression methods to detect publication bias, applied to this association and others, did not suggest that publication bias was present.
Relationship between QUS values and fracture
Lower values of the QUS parameters were associated with a significant increase of any subsequent fracture at any site (Table 2; Fig. 3). The age-adjusted RR for any fracture per each SD decrease ranged between 1.55 and 1.74 depending on the QUS parameter analyzed. The results were also significant for hip (Table 3; Fig. 4), forearm/wrist, and humeral fractures (Table 2). The age-adjusted relationships between QUS parameters and low-energy fractures are shown in Table 3. The RRs for this type of fracture were similar or slightly higher than for any fracture regardless the level of trauma. Thus, significant RRs of 1.74 (1.38–2.21), 1.73 (1.38–2.17), and 1.66 (1.39–1.99) for each SD decrease in BUA, SOS, and QUI/SI, respectively, were found (Table 3).
We finally analyzed the magnitude of the association of overall fractures risk in those studies that, in addition to QUS, included a BMD measurement by DXA. In the five studies where these data were available, which comprised nearly 14,000 patients, the relationship between BMD measured by DXA and fractures was comparable with BUA [RRBMD = 1.60 (1.22–2.05) versus RRBUA = 1.50 (1.26–1.77)]. Data for SOS were similar, but based on a smaller number of cases (∼7000) [RRSOS = 1.77 (1.17–2.68) versus RRBMD 1.74 (1.50–2.02); Table 4].
Meta-analysis of 14 prospective studies, which included nearly 43,700 individuals, showed the relationship between low QUS values and increased fracture risk independently of age and other covariates. Depending on the QUS parameter, the type of fracture, and the ultrasound measurement site, the estimated relative risk ranged between 1.23 and 1.94 for each SD decrease in QUS measurements, being statistically significant in all circumstances.
QUS methods have been introduced in clinical practice for the assessment of skeletal status in the last 15 years.(15) Its low cost and portability make QUS an attractive technology for assessing risk of fracture in larger populations than may be suitable for axial DXA. Moreover, the lack of exposure to ionizing radiation enhances the patient's acceptance of the test, and the operation of the equipment because less demanding regulations and space. Bone ultrasound parameters might measure different physical properties of bone to provide complementary information about fracture risk beyond BMD.(6) However, their potential clinical applications have not been fully validated, and several approaches have been proposed.(6,45,46) These include the diagnosis of osteoporosis, monitoring of skeletal changes caused by therapeutic interventions or disease progression, fracture risk assessment, or as a prescreening tool for identifying subjects who would be referred to further diagnostic work-up using DXA or biochemical markers of bone turnover. Whereas some of these strategies are still in early stages of research, the present systematic review of prospective fracture studies clearly support the concept that QUS, mainly at the heel, is a valuable tool to detect an increased risk of fracture. These results also confirm the findings of numerous cross-sectional studies that have shown that QUS values are generally lower in patients with fragility fractures.(8–14)
The association with an increased risk of fracture we have found (RR of 1.55–1.74 for each 1 SD reduction in QUS parameters for any fracture regardless of trauma and 1.66–1.74 for any low-trauma fracture) shows that the performance of QUS devices is similar to peripheral or axial DXA for these same categories of fractures reported in meta-analysis and large series(4,17,47,48) or in our own analysis (Table 4). However, it was inferior to BMD measurement at the femoral neck with DXA as a risk factor of hip fractures, as reported in a recent review of 12 cohorts, where the risk ratio increased by 2.94 in women for each SD decrease in hip BMD.(4)
It should not be concluded from this work that QUS may substitute DXA. Axial DXA is still considered the “gold standard” method for osteoporosis diagnosis or fracture assessment because its superior precision and capability to accurate measure BMD at axial sites and because more clinical experience and longer follow-up data have been accumulated in the last decades. Moreover, the current World Health Organization diagnosis criteria of osteoporosis, and the main treatment trials in osteoporosis have selected the patient population based on DXA values, making this technique the most validated to support therapy interventions with drugs. However, QUS may constitute a valid alternative to evaluate fracture risk in those circumstances and geographies where DXA equipment are not easily accessible. As with DXA, one important factor that should be taken into consideration when using and interpreting QUS results, is that the different equipment are not comparable, and that the results obtained on one device cannot de directly translated to other different QUS devices, especially if different QUS parameters are measured, as with forearm or finger equipment. The available devices show a big technological diversity and appropriate standardization approaches are eagerly needed. Moreover, the precision of QUS measurements is normally lower than with DXA, with %CV ∼ 2–3%, and varies depending on the parameter measured, the site, and the device used.(49)
In any case, it should be stressed that the assessment of fracture risk can not rely solely on the basis of bone properties, measured by DXA, QUS, or any other method, but in the integration of the skeletal and clinical risk factors in an individual for a given period of time, to express the fracture risk in absolute terms.(50,51) In fact, low QUS values may be considered like an additional independent risk factor for fractures, more than a diagnostic tool to establish whether the patient may be qualified as osteoporotic, osteopenic, or normal. Bone QUS may be also used as a prescreening tool for identifying subjects at highest risk who should be subjected to further risk assessment with bone densitometry, biochemical markers of bone turnover, or alternative techniques to evaluate the bone status.(46)
The two principal variables that are measured with QUS are the SOS passing through the bone, and the attenuation of the ultrasound wave (BUA). These variables are related to several material properties of the tissue as BMD, trabecular orientation, the proportion of trabecular and cortical bone, the composition of the organic and inorganic components, and fatigue damage of the bone. The velocity of an ultrasound wave depends mainly on the material properties of the tissues through which it is propagating, specifically of the BMD and the Young's modulus (a measure of resistance to deformation). The main mechanism of attenuation or energy lost of the ultrasound wave when propagated through the bone tissue is believed to be scattering and absorption.(52) Most of the manufacturers have developed derived parameters from BUA and SOS, such as the so-called “stiffness index” (SI) or the “quantitative ultrasound index” (QUI). These parameters are not directly related to biomechanical properties of bone, but they improve the standardized CV of SOS or BUA alone and compensate for heel temperature variations.(53) Moreover, QUI is more strongly correlated to actual heel BMD obtained with DXA. These derived parameters have been more recently introduced, and the number of prospective studies that have tested them is lower. However, our results show that the decrease in QUI/stiffness measurements shows the better relationship with increased fracture risk for overall fractures, regardless the level of trauma, and hip, forearm/wrist and humerus fractures analyzed separately. The relative risk for hip fracture associated with 1 SD decrease in heel QUI/stiffness in our analysis was 1.94 (1.46–2.59). This value represents a stronger association than the risk of hip fracture observed with 1 SD decrease in spinal BMD measured by DXA according to the results reported in a meta-analysis of prospective studies involving bone densitometry [RR = 1.6 (95% CI = 1.2–2.2)].(47) Our results also show the use of these parameters to evaluate the risk of forearm/wrist and humerus fractures, two common fracture sites associated to low bone mass,(54) and very prevalent in the elderly population. In contrast, we found very limited data in prospective studies to evaluate the capacity of QUS to assess vertebral fracture risk. Thus, the bulk evidence of the relationship of QUS and vertebral fracture risk remains supported by retrospective studies,(10,55–58) which are less accurate and with limited external validity.
The results of our systematic review are based in the assessment of nearly 47,300 individuals and ∼124,100 person-years of prospective observation. These numbers are in the same order of magnitude than meta-analysis of BMD measured with DXA and fracture prediction, where 90,000(47) and 168,366(4) person-years data have been reported. However, some limitations for its external validity should be noted. First, given the very few published studies and the small populations included in prospective cohorts, we were not able to perform a meta-analysis of the fracture risk associated to QUS measured at nonheel sites. Measurements of QUS at the patella and phalanx are restricted to a single variable, namely velocity of sound or its variations, and although these equipment are able to discriminate between fractured and nonfractured patients in most,(10,11,57,59) but not all,(58,60,61) cross-sectional studies, the magnitude of the relationship is normally lower than with BUA, and there is a need for long-term prospective studies with larger patient populations to evaluate the capability of these equipment to assess fracture risk. Second, given the low number of studies in men, the results of this meta-analysis apply only to women. In the largest prospective study in men, Khaw et al.(42) showed that each SD decrease in BUA and SOS values significantly increased the risk of future fracture by 87% and 65%, respectively, in 6485 men, but this analysis was based on 33 fractures only. Thus, until further evidence is available in men, the applicability of our results to male populations should be questioned. Moreover, although the race of the patients was usually not specified, given the geographies where most of these prospective studies were carried out, the results should be interpreted with caution for black women. It has been recently shown that the relationship between BMD and incident nonspinal fractures is significantly lower in black women from the SOF study,(62) and it is uncertain whether this may also be true for QUS. Finally, the results are mainly applicable to elderly women ⩾60 years of age.
In summary, this systematic review has shown that there is strong evidence from prospective studies that QUS measurements, BUA, and SOS, and their derived parameters (QUI/stiffness), are significantly associated with fracture risk, mainly among older women. The strength of the association of QUS with nonspinal fractures is similar to axial or peripheral DXA, but it is inferior to the association between BMD measured at the hip and hip fractures. Furthermore, lower heel QUS values are associated with forearm/wrist and humerus fractures. QUS can be considered a simpler and valid alternative to DXA to assess future fracture risk at nonspinal sites.
This study was supported by an unrestrictive research grant from the Medical Research Department, Eli Lilly and Company, Madrid, Spain.
- 31998 Osteoporosis: Review of the evidence for prevention, diagnosis and treatment and cost-effectiveness analysis. Osteoporos Int 8(Suppl 4): S1–S88., , , , , ,
- 5Osteoporosis in Europe 2005 Indicators of progress. Available online at http://www.osteofound.org/advocacy_policy/eu_policy_project/pdf/indicators_of_progress.pdf. Accessed February 4, 2006.
- 6for the International Quantitative Ultrasound Consensus Group 1997 Quantitative ultrasound techniques for the assessment of osteoporosis: Expert agreement on current status. J Bone Miner Res 12: 1280–1288.,
- 102004 Association of five quantitative ultrasound devices and bone densitometry with osteoporotic vertebral fractures in a population-based sample: The OPUS Study. J Bone Miner Res 19: 782–793., , , , , , , , , , , ,Direct Link:
- 151999 Clinical applications of quantitative ultrasound. NjehCF, HansD, FuerstT, GlüerCC, GenantHK (eds.) Quantitative Ultrasound: Assessment of Osteoporosis and Bone Status. Martin Dunitz, London, UK, 284–297.
- 222005 STROBE Statement: STrengthening the Reporting of OBservational studies in Epidemiology. Version 3Available online at http://www.strobe-statement.org/Checkliste.html. Accessed October 18, 2005., , ,
- 402004 Rôle des ultrasons osseoux pour la prédiction du risque de fracture de la hanche chez la femme âgée de 70 ans ou plus: Résults de l'étude SEMOF et comparaison avec les données de la littérature. Rev Med Suisse Rom 124: 59–62., , ,
- 412003 Prediction of non vertebral fracture risk by heel ultrasound in a population of 7,585 swiss women aged 70 to 80 years. Calcif Tissue Int 72: O43., , ,
- 432005 Prediction of non-vertebral fracture risk in 5201 postmenopausal women by quantitative ultrasound of the calcaneus: 36-month final results from the ECOSAP prospective study. J Bone Miner Res 20: S1; S67., , , , , , , , , ,
- 491999 Calcaneal quantitative ultrasound systems: Gel-coupled. NjehCF, HansD, FuerstT, GlüerCC, GenantHK (eds.) Quantitative Ultrasound: Assessment of Osteoporosis and Bone Status. Martin Dunitz, London, UK, 125–144., ,
- 531998 Stiffness compensates for effects in ultrasonometry. J Clin Densitom 1: 104., , ,
- 572002 Discriminatory ability of quantitative ultrasound parameters and bone mineral density in a population-based sample of postmenopausal women with vertebral fractures: Results of the Basel Osteoporosis Study. J Bone Miner Res 17: 321–330., , , , , , ,