Cysteine at Position 217 in the Intracellular Loop 1 Plays a Critical Role in Human PTH Receptor Type 1 Membrane Translocation and Function

Authors

  • Beena E Thomas,

    1. Department of Periodontology and Oral Biology, Boston University School of Dental Medicine, Boston, Massachusetts, USA
    2. Department of Orthopedic Surgery, Boston University School of Medicine, Boston, Massachusetts, USA
    Search for more papers by this author
  • Angela Wittelsberger,

    1. Department of Periodontology and Oral Biology, Boston University School of Dental Medicine, Boston, Massachusetts, USA
    2. Department of Orthopedic Surgery, Boston University School of Medicine, Boston, Massachusetts, USA
    Search for more papers by this author
  • Iwona Woznica,

    1. Department of Periodontology and Oral Biology, Boston University School of Dental Medicine, Boston, Massachusetts, USA
    Search for more papers by this author
  • Mo-Ying Hsieh,

    1. Department of Periodontology and Oral Biology, Boston University School of Dental Medicine, Boston, Massachusetts, USA
    Search for more papers by this author
  • Paul Monaghan,

    1. Department of Periodontology and Oral Biology, Boston University School of Dental Medicine, Boston, Massachusetts, USA
    Search for more papers by this author
  • Byung-Kwon Lee,

    1. Department of Aerospace and Mechanical Engineering, Boston University College of Engineering, Boston, Massachusetts, USA
    Search for more papers by this author
  • Michael Rosenblatt MD

    Corresponding author
    1. Department of Periodontology and Oral Biology, Boston University School of Dental Medicine, Boston, Massachusetts, USA
    • Department of Physiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA
    Search for more papers by this author

  • The authors state that they have no conflicts of interest.

Abstract

PTHR1 mutants lacking endogenous cysteines in transmembrane and intracellular domains were generated. Mutant receptors were tested for their biological activities and mRNA and cell surface expression levels. C217 in intracellular loop 1 was determined to play a critical role in cell surface translocation and function of the receptor.

Introduction: Elucidating the role of different domains of PTH receptor 1 (PTHR1) is essential for understanding the mechanism of ligand–receptor interactions. Here we present a study directed at determining the importance of cysteine residues present in the intracellular and transmembrane (TM) domains of the receptor.

Materials and Methods: Mutant receptors were generated by site-directed mutagenesis. Biological activities were characterized by adenylyl cyclase and competition binding assays. RT-PCR, ELISA, and immunofluorescence microscopy were carried out to determine receptor mRNA and protein expression levels.

Results: Mutations C460L and C462L in TM7, C568L in the C-terminal intracellular domain of the receptor, and removal of C397 in intracellular loop (ICL)3 by insertion of cleavage sites for Factor Xa did not affect binding affinity of PTH or agonist-induced adenylyl cyclase activity, although maximal responses (ICmax and ECmax) were decreased. However, mutations C217L in ICL1 or both C217L and C568L simultaneously resulted in a decrease in binding and loss of adenylyl cyclase activity. RT-PCR results showed that the observed changes in binding and activity were not caused by changes in mRNA expression. Next, we determined cell surface and total expression of the wildtype and mutant receptors by ELISA. We found that mutations of C460/C462 to L moderately decreased transfer of receptors to the cell surface. However, mutation of C217 to L in the ICL1 drastically reduced cell surface expression. Immunofluorescence and confocal microscopy studies confirmed reduced cell surface expression of receptors containing the C217L mutation. Similar results were obtained when replacing C217 and C460/C462 of the receptor with A instead of L.

Conclusions: Our studies indicate that the cysteine at position 217 in ICL1 plays a critical role in translocation to the cell surface and biological function of PTHR1.

Ancillary