1523-4681/asset/olbannerleft.gif?v=1&s=d7e4c0e37904a489128d3a4e58ba94214db307a9)
1523-4681/asset/olbannerright.gif?v=1&s=854ee0e4d351ead9faaca8bfab3e50b1c7c9d03d)
Original Article
You have full text access to this OnlineOpen article
Deficiency of annexins A5 and A6 induces complex changes in the transcriptome of growth plate cartilage but does not inhibit the induction of mineralization
Article first published online: 18 DEC 2009
DOI: 10.1359/jbmr.090710
Copyright © 2010 American Society for Bone and Mineral Research
Additional Information
How to Cite
Belluoccio, D., Grskovic, I., Niehoff, A., Schlötzer-Schrehardt, U., Rosenbaum, S., Etich, J., Frie, C., Pausch, F., Moss, S. E., Pöschl, E., Bateman, J. F. and Brachvogel, B. (2010), Deficiency of annexins A5 and A6 induces complex changes in the transcriptome of growth plate cartilage but does not inhibit the induction of mineralization. J Bone Miner Res, 25: 141–153. doi: 10.1359/jbmr.090710
Publication History
- Issue published online: 20 JAN 2010
- Article first published online: 18 DEC 2009
- Manuscript Accepted: 1 JUL 2009
- Manuscript Revised: 24 MAR 2009
- Manuscript Received: 26 JAN 2009
References
- 1, , , et al. The roles of annexins and alkaline phosphatase in mineralization process. Acta Biochim Pol. 2003; 50: 1019–1038.
- 2
- 3, , , , . Calcium influx through annexin V ion channels into large unilamellar vesicles measured with fura-2. FEBS Lett. 1993; 317: 131–134.
- 4, . Remodelling of collagen types I, II and X and calcification of human fetal cartilage. Bone Miner. 1992; 18: 107–117.
- 5, , , , , . Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood. 1994; 84: 1415–1420.
- 6, , . Kinetic analysis of mineral formation during in vitro modeling of matrix vesicle mineralization: effect of annexin A5, phosphatidylserine, and type II collagen. Anal Biochem. 2007; 367: 159–166.
- 7, , . In vitro modeling of matrix vesicle nucleation: synergistic stimulation of mineral formation by annexin A5 and phosphatidylserine. J Biol Chem. 2007; 282: 26035–26045.
- 8, . Collagen/annexin V interactions regulate chondrocyte mineralization. J Biol Chem. 2008; 283: 10310–10317.
- 9, , , et al. Isolated Anxa5(+)/Sca-1(+) perivascular cells from mouse meningeal vasculature retain their perivascular phenotype in vitro and in vivo. Exp Cell Res. 2007; 313: 2730–2743.
- 10, , , et al. Perivascular cells expressing annexin A5 define a novel mesenchymal stem cell-like population with the capacity to differentiate into multiple mesenchymal lineages. Development. 2005; 132: 2657–2668.
- 11, . Angiogenesis and pericytes in the initiation of ectopic calcification. Circ Res. 2005; 96: 930–938.
- 12, , , . The roles of annexins and types II and X collagen in matrix vesicle-mediated mineralization of growth plate cartilage. J Biol Chem. 2000; 275: 35577–35583.
- 13, , , , . Immunological development and cardiovascular function are normal in annexin VI null mutant mice. Mol Cell Biol. 1999; 19: 8028–8032.
- 14, , , et al. Annexin II regulates fibrin homeostasis and neoangiogenesis in vivo. J Clin Invest. 2004; 113: 38–48.
- 15, , , et al. Annexin A5 is not essential for skeletal development. Mol Cell Biol 2003; 23: 2907–2913.
- 16, , , et al. Abnormal compartmentalization of cartilage matrix components in mice lacking collagen X: implications for function. J Cell Biol. 1997; 136: 459–471.
- 17. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72: 248–254.
- 18, , , , , . Transgenic mice expressing D469delta mutated cartilage oligomeric matrix protein (COMP) show growth plate abnormalities and sternal malformations. Matrix Biol. 2008; 27: 67–85.
- 19
- 20, , , . Ablation of collagen IX and COMP disrupts epiphyseal cartilage architecture. Matrix Biol. 2008; 27: 306–318.
- 21, , , et al. Altered integration of matrilin-3 into cartilage extracellular matrix in the absence of collagen IX. Mol Cell Biol. 2005; 25: 10465–10478.
- 22, , , , . Cartilage oligomeric matrix protein (COMP) is an abundant component of tendon. FEBS Lett. 1994; 354: 237–240.
- 23, , , et al. Immunolocalization of type X collagen in normal fetal and adult osteoarthritic cartilage with monoclonal antibodies. Matrix Biol. 1996; 15: 231–238.
- 24, , , . A microarray approach for comparative expression profiling of the discrete maturation zones of mouse growth plate cartilage. Biochim Biophys Acta. 2008; 1779: 330–340.
- 25, , , , , . Amplified RNA synthesized from limited quantities of heterogeneous cDNA. Proc Natl Acad Sci USA. 1990; 87: 1663–1667.
- 26, . Analysis of relative gene expression data using real-time quantitative PCR and the 2(–δδC(T)) method. Methods. 2001; 25: 402–408.
- 27. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001; 29: 45.
- 28, , , et al. Proteome analysis of matrix vesicles isolated from femurs of chicken embryo. Proteomics. 2008; 8: 192–205.Direct Link:
- 29, , , , . Annexin VIII is differentially expressed by chondrocytes in the mammalian growth plate during endochondral ossification and in osteoarthritic cartilage. J Bone Miner Res. 2002; 17: 1851–1858.Direct Link:
- 30. The complexities of skeletal biology. Nature. 2003; 423: 316–318.
- 31
- 32, , , . Extracellular matrix mineralization is regulated locally: different roles of two gla-containing proteins. J Cell Biol. 2004; 165: 625–630.
- 33. Annexins: their role in cartilage mineralization. Front Biosci. 2005; 10: 576–581.
- 34, . Molecular phylogeny of annexins and identification of a primitive homologue in Giardia lamblia. Mol Biol Evol. 1995; 12: 967–979.
- 35, , , . Role of annexin 1 gene expression in mouse craniofacial bone development. Birth Defects Res A Clin Mol Teratol. 2007; 79: 524–532.Direct Link:
- 36, , , et al. The crystal structure of annexin A8 is similar to that of annexin A3. J Mol Biol. 2005; 345: 1131–1139.
- 37, , , et al. Interactions of benzodiazepine derivatives with annexins. J Biol Chem. 1998; 273: 2885–2894.
- 38, , , et al. Haploinsufficiency of Anx7 tumor suppressor gene and consequent genomic instability promotes tumorigenesis in the Anx7(+/–) mouse. Proc Natl Acad Sci USA. 2003; 100: 14287–14292.
- 39, , , et al. Defects in inositol 1,4,5-trisphosphate receptor expression, Ca2+ signaling, and insulin secretion in the anx7(+/–) knockout mouse. Proc Natl Acad Sci USA. 1999; 96: 13783–13788.
- 40, , , , . Absence of platelet-activating factor receptor protects mice from osteoporosis following ovariectomy. J Clin Invest. 2004; 114: 85–93.
- 41, , . Annexins: linking Ca2+ signalling to membrane dynamics. Nat Rev Mol Cell Biol. 2005; 6: 449–461.
- 42, , , et al. Annexin VI is associated with calcium-sequestering organelles. J Cell Biochem. 1991; 46: 78–85.Direct Link:
- 43, , , , . Annexin V disruption impairs mechanically induced calcium signaling in osteoblastic cells. Bone. 2004; 35: 656–663.