Original Article

You have full text access to this OnlineOpen article

Capacity of omega-3 fatty acids or eicosapentaenoic acid to counteract weightlessness-induced bone loss by inhibiting NF-κB activation: From cells to bed rest to astronauts

  1. Sara R Zwart1,*,
  2. Duane Pierson2,
  3. Satish Mehta3,
  4. Steve Gonda4,
  5. Scott M Smith4

Article first published online: 14 DEC 2009

DOI: 10.1359/jbmr.091041

Issue

Journal of Bone and Mineral Research

Journal of Bone and Mineral Research

Volume 25, Issue 5, pages 1049–1057, May 2010

Additional Information

How to Cite

Zwart, S. R., Pierson, D., Mehta, S., Gonda, S. and Smith, S. M. (2010), Capacity of omega-3 fatty acids or eicosapentaenoic acid to counteract weightlessness-induced bone loss by inhibiting NF-κB activation: From cells to bed rest to astronauts. J Bone Miner Res, 25: 1049–1057. doi: 10.1359/jbmr.091041

Author Information

  1. 1

    Universities Space Research Association, Houston, TX, USA

  2. 2

    NASA Johnson Space Center, Habitability and Environmental Factors Division, Houston, TX, USA

  3. 3

    Enterprise Advisory Services, Inc., Houston, TX, USA

  4. 4

    NASA Johnson Space Center, Human Adaptation and Countermeasures Division, Houston, TX, USA

*Universities Space Research Association, NASA Johnson Space Center, Human Adaptation and Countermeasures Division (SK3), 2101 NASA Parkway, Houston, TX 77058, USA.

Publication History

  1. Issue published online: 30 APR 2010
  2. Article first published online: 14 DEC 2009
  3. Accepted manuscript online: 27 JAN 2010 12:00AM EST
  4. Manuscript Accepted: 22 OCT 2009
  5. Manuscript Revised: 14 OCT 2009
  6. Manuscript Received: 12 AUG 2009

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (219K)

References

  • 1
    Sibonga JD, Cavanagh PR, Lang TF, et al. Adaptation of the skeletal system during long-duration spaceflight. Clin Rev Bone Miner Metab. 2008; 5: 249–261.
  • 2
    LeBlanc AD, Spector ER, Evans HJ, Sibonga JD. Skeletal responses to space flight and the bed rest analog: a review. J Musculoskelet Neuronal Interact. 2007; 7: 33–47.
  • 3
    Trappe S, Costill D, Gallagher P, et al. Exercise in space: human skeletal muscle after 6 months aboard the International Space Station. J Appl Physiol. 2009; 106: 1159–1168.
  • 4
    Durham WJ, Li YP, Gerken E, et al. Fatiguing exercise reduces DNA binding activity of NF-κB in skeletal muscle nuclei. J Appl Physiol. 2004; 97: 1740–1745.
  • 5
    Judge AR, Koncarevic A, Hunter RB, Liou HC, Jackman RW, Kandarian SC. Role for IκBα, but not c-Rel, in skeletal muscle atrophy. Am J Physiol Cell Physiol. 2007; 292: C372–382.
  • 6
    Hunter RB, Stevenson E, Koncarevic A, Mitchell-Felton H, Essig DA, Kandarian SC. Activation of an alternative NF-κB pathway in skeletal muscle during disuse atrophy. FASEB J. 2002; 16: 529–538.
  • 7
    Farid M, Reid MB, Li YP, Gerken E, Durham WJ. Effects of dietary curcumin or N-acetylcysteine on NF-κB activity and contractile performance in ambulatory and unloaded murine soleus. Nutr Metab. 2005; 2: 20–28.
  • 8
    Rahman MM, Bhattacharya A, Fernandes G. Docosahexaenoic acid is more potent inhibitor of osteoclast differentiation in RAW 264.7 cells than eicosapentaenoic acid. J Cell Physiol. 2008; 214: 201–209.
    Direct Link:
  • 9
    Fernandes G, Bhattacharya A, Rahman M, Zaman K, Banu J. Effects of n-3 fatty acids on autoimmunity and osteoporosis. Front Biosci. 2008; 13: 4015–4020.
  • 10
    Galliera E, Locati M, Mantovani A, Corsi MM. Chemokines and bone remodeling. Int J Immunopathol Pharmacol. 2008; 21: 485–491.
  • 11
    Tilg H, Moschen AR, Kaser A, Pines A, Dotan I. Gut, inflammation and osteoporosis: basic and clinical concepts. Gut. 2008; 57: 684–694.
  • 12
    Whitehouse AS, Smith HJ, Drake JL, Tisdale MJ. Mechanism of attenuation of skeletal muscle protein catabolism in cancer cachexia by eicosapentaenoic acid. Cancer Res. 2001; 61: 3604–3609.
  • 13
    Wigmore SJ, Barber MD, Ross JA, Tisdale MJ, Fearon KC. Effect of oral eicosapentaenoic acid on weight loss in patients with pancreatic cancer. Nutr Cancer. 2000; 36: 177–184.
  • 14
    Riediger ND, Othman RA, Suh M, Moghadasian MH. A systemic review of the roles of n-3 fatty acids in health and disease. J Am Diet Assoc. 2009; 109: 668–679.
  • 15
    Smith HJ, Lorite MJ, Tisdale MJ. Effect of a cancer cachectic factor on protein synthesis/degradation in murine C2C12 myoblasts: modulation by eicosapentaenoic acid. Cancer Res. 1999; 59: 5507–5513.
  • 16
    Alexander JW. Immunonutrition: the role of omega-3 fatty acids. Nutrition. 1998; 14: 627–633.
  • 17
    Sun D, Krishnan A, Zaman K, Lawrence R, Bhattacharya A, Fernandes G. Dietary n-3 fatty acids decrease osteoclastogenesis and loss of bone mass in ovariectomized mice. J Bone Miner Res. 2003; 18: 1206–1216.
    Direct Link:
  • 18
    Fernandes G, Lawrence R, Sun D. Protective role of n-3 lipids and soy protein in osteoporosis. Prostaglandins Leukot Essent Fatty Acids. 2003; 68: 361–372.
  • 19
    Yaqoob P, Calder PC. N-3 polyunsaturated fatty acids and inflammation in the arterial wall. Eur J Med Res. 8: 2003; 337–354.
  • 20
    Micallef MA, Munro IA, Garg ML. An inverse relationship between plasma n-3 fatty acids and C-reactive protein in healthy individuals. Eur J Clin Nutr (May 19). 2009; 1–5.
  • 21
    Moon DO, Kim KC, Jin CY, et al. Inhibitory effects of eicosapentaenoic acid on lipopolysaccharide-induced activation in BV2 microglia. Int Immunopharmacol. 2007; 7: 222–229.
  • 22
    Tisdale MJ. The ubiquitin-proteasome pathway as a therapeutic target for muscle wasting. J Support Oncol. 2005; 3: 209–217.
  • 23
    Kim HH, Lee Y, Eun HC, Chung JH. Eicosapentaenoic acid inhibits TNF-α-induced matrix metalloproteinase-9 expression in human keratinocytes, HaCaT cells. Biochem Biophys Res Comm. 2008; 368: 343–349.
  • 24
    Camandola S, Leonarduzzi G, Musso T, et al. Nuclear factor κB is activated by arachidonic acid but not by eicosapentaenoic acid. Biochem Biophys Res Comm. 1996; 229: 643–647.
  • 25
    Rucci N, Migliaccio S, Zani BM, Taranta A, Teti A. Characterization of the osteoblast-like cell phenotype under microgravity conditions in the NASA-approved Rotating Wall Vessel bioreactor (RWV). J Cell Biochem. 2002; 85: 167–179.
    Direct Link:
  • 26
    Molnar G, Schroedl NA, Gonda SR, Hartzell CR. Skeletal muscle satellite cells cultured in simulated microgravity. In Vitro Cell Dev Biol Anim. 1997; 33: 386–391.
  • 27
    Pavy-Le Traon A, Heer M, Narici MV, Rittweger J, Vernikos J. From space to earth: advances in human physiology from 20 years of bed rest studies. (1986–2006). Eur J Appl Physiol. 2007; 101: 143–194.
  • 28
    Markwell MA, Haas SM, Bieber LL, Tolbert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978; 87: 206–210.
  • 29
    Zwart SR, Lewis BJ. Optimization of detection and quantification of proteins on membranes in very high and very low abundance using avidin and streptavidin. Methods Mol Biol. 2008; 418: 25–34.
  • 30
    Mehta SK, Kaur I, Grimm EA, Smid C, Feeback DL, Pierson DL. Decreased non-MHC-restricted (CD56+) killer cell cytotoxicity after spaceflight. J Appl Physiol. 2001; 91: 1814–1818.
  • 31
    Stowe RP, Sams CF, Mehta SK, et al. Leukocyte subsets and neutrophil function after short-term spaceflight. J Leuk Biol. 1999; 65: 179–186.
  • 32
    Bharti AC, Donato N, Aggarwal BB. Curcumin (diferuloylmethane) inhibits constitutive and IL-6-inducible STAT3 phosphorylation in human multiple myeloma cells. J Immunol. 2003; 171: 3863–3871.
  • 33
    Smith SM, Davis-Street JE, Rice BL, Nillen JL, Gillman PL, Block G. Nutritional status assessment in semiclosed environments: ground-based and space flight studies in humans. J Nutr. 2001; 131: 2053–2061.
  • 34
    Smith SM, Zwart SR, Block G, Rice BL, Davis-Street JE. The nutritional status of astronauts is altered after long-term space flight aboard the International Space Station. J Nutr. 2005; 135: 437–443.
  • 35
    Soller BR, Cabrera M, Smith SM, Sutton JP. Smart medical systems with application to nutrition and fitness in space. Nutrition. 2002; 18: 930–936.
  • 36
    Meck JV, Dreyer S, Warren L. Long-duration head-down bed rest: Project overview, vital signs, and fluid balance. Aviat Space Environ Med. 2009; 80 (5 Supplement, ): A1–A8.
  • 37
    Zwart SR, Oliver SM, Fesperman JV, et al. Nutritional status assessment before, during, and after long-duration head-down bed rest. Aviat Space Environ Med. 2009; 80: A15–A22.
  • 38
    Inniss AM, Rice BL, Smith SM. Dietary support of long-duration head-down bed rest. Aviat Space Environ Med. 2009; 80: A9–A14.
  • 39
    Schakel SF, Sievert YA, Buzzard IM. Sources of data for developing and maintaining a nutrient database. J Am Diet Assoc. 1988; 88: 1268–1271.
  • 40
    Spector ER, Smith SM, Sibonga JD. Skeletal effects of long-duration head-down bed rest. Aviat Space Environ Med. 2009; 80: A23–A28.
  • 41
    Kang JX, Weylandt KH. Modulation of inflammatory cytokines by omega-3 fatty acids. Subcell Biochem. 2008; 49: 133–143.
  • 42
    Chopra V, Fadl AA, Sha J, Chopra S, Galindo CL, Chopra AK. Alterations in the virulence potential of enteric pathogens and bacterial-host cell interactions under simulated microgravity conditions. J Toxicol Environ Health A. 2006; 69: 1345–1370.
  • 43
    Bakos A, Varkonyi A, Minarovits J, Batkai L. Effect of simulated microgravity on human lymphocytes. J Gravit Physiol. 2001; 8: P69–70.
  • 44
    Ulbrich C, Westphal K, Baatout S, et al. Effects of basic fibroblast growth factor on endothelial cells under conditions of simulated microgravity. J Cell Biochem. 2008; 104: 1324–1341.
    Direct Link:
  • 45
    Granet C, Boutahar N, Vico L, Alexandre C, Lafage-Proust MH. MAPK and SRC-kinases control EGR-1 and NF-κB inductions by changes in mechanical environment in osteoblasts. Biochem Biophys Res Commun. 2001; 284: 622–631.
  • 46
    Kwon O, Tranter M, Jones WK, Sankovic JM, Banerjee RK. Differential translocation of nuclear factor-κB in a cardiac muscle cell line under gravitational changes. J Biomech Eng. 2009; 131: 064503–064507.
  • 47
    Wise KC, Manna SK, Yamauchi K, et al. Activation of nuclear transcription factor-κB in mouse brain induced by a simulated microgravity environment. In Vitro Cell Dev Biol Anim. 2005; 41: 118–123.
  • 48
    Bar-Shai M, Carmeli E, Reznick AZ. The role of NF-κB in protein breakdown in immobilization, aging, and exercise: from basic processes to promotion of health. Ann N Y Acad Sci. 2005; 1057: 431–447.
    Direct Link:
  • 49
    Visioli F, Rise P, Barassi MC, Marangoni F, Galli C. Dietary intake of fish vs. formulations leads to higher plasma concentrations of n-3 fatty acids. Lipids. 2003; 38: 415–418.
  • 50
    Rousseau JH, Kleppinger A, Kenny AM. Self-reported dietary intake of omega-3 fatty acids and association with bone and lower extremity function. J Am Geriatr Soc. 2009; 57: 1781–1788.
    Direct Link:
  • 51
    Griel AE, Kris-Etherton PM, Hilpert KF, Zhao G, West SG, Corwin RL. An increase in dietary n-3 fatty acids decreases a marker of bone resorption in humans. Nutr J. 2007; 6: 2–10.
  • 52
    Bhattacharya A, Rahman M, Sun D, Fernandes G. Effect of fish oil on bone mineral density in aging C57BL/6 female mice. J Nutr Biochem. 2007; 18: 372–379.
  • 53
    Tisdale MJ. Mechanisms of cancer cachexia. Physiol Rev. 2009; 89: 381–410.
  • 54
    Fearon KC, Barber MD, Moses AG, et al. Double-blind, placebo-controlled, randomized study of eicosapentaenoic acid diester in patients with cancer cachexia. J Clin Oncol. 2006; 24: 3401–3407.
  • 55
    Khal J, Tisdale MJ. Downregulation of muscle protein degradation in sepsis by eicosapentaenoic acid (EPA). Biochem Biophys Res Commun. 2008; 375: 238–240.
  • 56
    Turner ND, Braby LA, Ford J, Lupton JR. Opportunities for nutritional amelioration of radiation-induced cellular damage. Nutrition. 2002; 18: 904–912.
  • 57
    Davidson LA, Nguyen DV, Hokanson RM, et al. Chemopreventive n-3 polyunsaturated fatty acids reprogram genetic signatures during colon cancer initiation and progression in the rat. Cancer Res. 2004; 64: 6797–6804.
  • 58
    Chapkin RS, Davidson LA, Ly L, Weeks BR, Lupton JR, McMurray DN. Immunomodulatory effects of (n-3) fatty acids: putative link to inflammation and colon cancer. J Nutr. 2007; 137: 200S–204S.
  • 59
    Hong MY, Bancroft LK, Turner ND, et al. Fish oil decreases oxidative DNA damage by enhancing apoptosis in rat colon. Nutr Cancer. 2005; 52: 166–175.
  • 60
    Sanders LM, Henderson CE, Hong MY, et al. An increase in reactive oxygen species by dietary fish oil coupled with the attenuation of antioxidant defenses by dietary pectin enhances rat colonocyte apoptosis. J Nutr. 2004; 134: 3233–3238.
  • 61
    Vanamala J, Glagolenko A, Yang P, et al. Dietary fish oil and pectin enhance colonocyte apoptosis in part through suppression of PPARδ/PGE2 and elevation of PGE3. Carcinogenesis. 2008; 29: 790–796.
View Full Article (HTML) Get PDF (219K)