• 1
    Brockstedt H, Kassem M, Eriksen EF, Mosekilde L, Melsen F 1993 Age- and sex-related changes in iliac cortical bone mass and remodeling. Bone 14:681691.
  • 2
    Kragstrup J, Melsen F, Mosekilde L 1983 Thickness of bone formed at remodeling sites in normal human iliac trabecular bone: Variations with age and sex. Metab Bone Dis Relat Res 5:1721.
  • 3
    Eriksen EF, Hodgson SF, Eastell R, Cedel SL, O'Fallon WM, Riggs BL 1990 Cancellous bone remodeling in type I (postmenopausal) osteoporosis: Quantitative assessment of rates of formation, resorption, and bone loss at tissue and cellular levels. J Bone Miner Res 5:311319.
  • 4
    Cohen-Solal ME, Shih MS, Lundy MW, Parfitt AM 1991 A new method for measuring cancellous bone erosion depth: Application to the cellular mechanisms of bone loss in postmenopausal osteoporosis. J Bone Miner Res 6:13311338.
  • 5
    Parfitt AM 1990 Bone forming cells in clinical conditions. In: HallBK (ed.) The Osteoblast and Osteocyte. The Telford Press, London, UK, pp. 351426.
  • 6
    Friedenstein AJ 1976 Precursor cells of mechanocytes. Int Rev Cytol 47:327359.
  • 7
    Friedenstein AJ, Chailakhyan RK, Gerasimov UV 1987 Bone marrow osteogenic stem cells: In vitro cultivation and transplantation in diffusion chambers. Cell Tissue Kinet 20:263272.
  • 8
    Owen M 1988 Marrow stromal stem cells. J Cell Sci Suppl 10:6376.
  • 9
    Kassem M, Risteli L, Mosekilde L, Melsen F, Eriksen EF 1991 Formation of osteoblast-like cells from human mononuclear bone marrow cultures. APMIS 99:269274.
  • 10
    Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR 1999 Multilineage potential of adult human mesenchymal stem cells. Science 284:143147.
  • 11
    Rickard DJ, Kassem M, Hefferan TE, Sarkar G, Spelsberg TC, Riggs BL 1996 Isolation and characterization of osteoblast precursor cells from human bone marrow. J Bone Miner Res 11:312324.
  • 12
    Cheng SL, Yang JW, Rifas L, Zhang SF, Avioli LV 1994 Differentiation of human bone marrow osteogenic stromal cells in vitro: Induction of the osteoblast phenotype by dexamethasone. Endocrinology 134:277286.
  • 13
    Castro-Malaspina H, Gay RE, Resnick G, Kapoor N, Meyers P, Chiarieri D, McKenzie S, Broxmeyer HE, Moore MA 1980 Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny. Blood 56:289301.
  • 14
    Friedenstein AJ, Chailakhjan RK, Lalykina KS 1970 The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet 3:393403.
  • 15
    Egrise D, Martin D, Vienne A, Neve P, Schoutens A 1992 The number of fibroblastic colonies formed from bone marrow is decreased and the in vitro proliferation rate of trabecular bone cells increased in aged rats. Bone 13:355361.
  • 16
    Quarto R, Thomas D, Liang CT 1995 Bone progenitor cell deficits and the age-associated decline in bone repair capacity. Calcif Tissue Int 56:123129.
  • 17
    Bergman RJ, Gazit D, Kahn AJ, Gruber H, McDougall S, Hahn TJ 1996 Age-related changes in osteogenic stem cells in mice. J Bone Miner Res 11:568577.
  • 18
    Kahn A, Gibbons R, Perkins S, Gazit D 1995 Age-related bone loss. A hypothesis and initial assessment in mice. Clin Orthop 313:6975.
  • 19
    Xu CX, Hendry JH, Testa NG, Allen TD 1983 Stromal colonies from mouse marrow: Characterization of cell types, optimization of plating efficiency and its effect on radiosensitivity. J Cell Sci 61:453466.
  • 20
    Brockbank KG, Ploemacher RE, van PC 1983 An in vitro analysis of murine hemopoietic fibroblastoid progenitors and fibroblastoid cell function during aging. Mech Ageing Dev 22:1121.
  • 21
    Nishida S, Endo N, Yamagiwa H, Tanizawa T, Takahashi HE 1999 Number of osteoprogenitor cells in human bone marrow markedly decreases after skeletal maturation. J Bone Miner Metab 17:171177.
  • 22
    D'Ippolito G, Schiller PC, Ricordi C, Roos BA, Howard GA 1999 Age-related osteogenic potential of mesenchymal stromal stem cells from human vertebral bone marrow. J Bone Miner Res 14:11151122.
  • 23
    Majors AK, Boehm CA, Nitto H, Midura RJ, Muschler GF 1997 Characterization of human bone marrow stromal cells with respect to osteoblastic differentiation. J Orthop Res 15:546557.
  • 24
    Oreffo RO, Bennett A, Carr AJ, Triffitt JT 1998 Patients with primary osteoarthritis show no change with ageing in the number of osteogenic precursors. Scand J Rheumatol 27:415424.
  • 25
    Oreffo RO, Bord S, Triffitt JT 1998 Skeletal progenitor cells and ageing human populations. Clin Sci 94:549555.
  • 26
    Gronthos S, Graves SE, Ohta S, Simmons PJ 1994 The STRO-1+ fraction of adult human bone marrow contains the osteogenic precursors. Blood 84:41644173.
  • 27
    Simmons PJ, Torok SB 1991 Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1. Blood 78:5562.
  • 28
    Stewart K, Walsh S, Screen J, Jefferiss CM, Chainey J, Jordan GR, Beresford JN 1999 Further characterization of cells expressing STRO-1 in cultures of adult human bone marrow stromal cells. J Bone Miner Res 14:13451356.
  • 29
    Stanford CM, Jacobson PA, Eanes ED, Lembke LA, Midura RJ 1995 Rapidly forming apatitic mineral in an osteoblastic cell line (UMR 106-01 BSP). J Biol Chem 270:94209428.
  • 30
    Vestergaard P, Hermann AP, Orskov H, Mosekilde L 1999 Effect of sex hormone replacement on the insulin-like growth factor system and bone mineral: A cross-sectional and longitudinal study in 595 perimenopausal women participating in the Danish Osteoporosis Prevention Study. J Clin Endocrinol Metab 84:22862290.
  • 31
    Stenderup K 1999 Effect of age and osteoporosis on growth potential of osteoprogenitor cells in human bone marrow, Candidate thesis, University of Aarhus, Aarhus, Denmark.
  • 32
    Kuznetsov SA, Friedenstein AJ, Robey PG 1997 Factors required for bone marrow stromal fibroblast colony formation in vitro. Br J Haematol 97:561570.
  • 33
    Friedenstein AJ, Latzinik NV, Gorskaya YF, Luria EA, Moskvina IL 1992 Bone marrow stromal colony formation requires stimulation by haemopoietic cells. Bone Miner 18:199213.
  • 34
    Smith JR, Whitney RG 1980 Intraclonal variation in proliferative potential of human diploid fibroblasts: Stochastic mechanism for cellular aging. Science 207:8284.
  • 35
    Gronthos S, Simmons PJ 1995 The growth factor requirements of STRO-1-positive human bone marrow stromal precursors under serum-deprived conditions in vitro. Blood 85:929940.
  • 36
    Owen ME, Cave J, Joyner CJ 1987 Clonal analysis in vitro of osteogenic differentiation of marrow CFU-F. J Cell Sci 87:731738.
  • 37
    Rodan GA, Noda M 1991 Gene expression in osteoblastic cells. Crit Rev Eukaryot Gene Expr 1:8598.
  • 38
    Bianco P, Gehron RP 2000 Marrow stromal stem cells. J Clin Invest 105:16631668.
  • 39
    Long MW, Ashcraft EK, Normalle D, Mann KG 1999 Age-related phenotypic alterations in populations of purified human bone precursor cells. J Gerontol A Biol Sci Med Sci 54:B54B62.
  • 40
    Koshihara Y, Hirano M, Kawamura M, Oda H, Higaki S 1991 Mineralization ability of cultured human osteoblast-like periosteal cells does not decline with aging. J Gerontol 46:B201B206.
  • 41
    Inoue K, Ohgushi H, Yoshikawa T, Okumura M, Sempuku T, Tamai S, Dohi Y 1997 The effect of aging on bone formation in porous hydroxyapatite: Biochemical and histological analysis. J Bone Miner Res 12:989994.
  • 42
    Liang CT, Barnes J, Seedor JG, Quartuccio HA, Bolander M, Jeffrey JJ, Rodan GA 1992 Impaired bone activity in aged rats: Alterations at the cellular and molecular levels. Bone 13:435441.
  • 43
    Eastell R, Delmas PD, Hodgson SF, Eriksen EF, Mann KG, Riggs BL 1988 Bone formation rate in older normal women: Concurrent assessment with bone histomorphometry, calcium kinetics, and biochemical markers. J Clin Endocrinol Metab 67:741748.
  • 44
    Kassem M, Brixen K, Mosekilde L, Eriksen EF 1994 Human marrow stromal osteoblast-like cells do not show reduced responsiveness to in vitro stimulation with growth hormone in patients with postmenopausal osteoporosis. Calcif Tissue Int 54:16.
  • 45
    Marie PJ, Sabbagh A, de VM, Lomri A 1989 Osteocalcin and deoxyribonucleic acid synthesis in vitro and histomorphometric indices of bone formation in postmenopausal osteoporosis. J Clin Endocrinol Metab 69:272279.
  • 46
    Pfeilschifter J, Diel I, Pilz U, Brunotte K, Naumann A, Ziegler R 1993 Mitogenic responsiveness of human bone cells in vitro to hormones and growth factors decreases with age. J Bone Miner Res 8:707717.
  • 47
    Kveiborg M, Flyvbjerg A, Rattan SI, Kassem MS 2000 Changes in the insulin-like growth factor-system may contribute to in vitro age-related impaired osteoblast functions. Exp Gerontol 35:10611074.