See also: T Hart et al (2014)
News & Views
The Good, the Bad, and the Ugly: in search of gold standards for assessing functional genetic screen quality
Article first published online: 1 JUL 2014
© 2014 The Authors. Published under the terms of the CC BY 4.0 license
This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Molecular Systems Biology
Volume 10, Issue 7, July 2014
How to Cite
Evers, B., Bernards, R. and Beijersbergen, R. L. (2014), The Good, the Bad, and the Ugly: in search of gold standards for assessing functional genetic screen quality. Molecular Systems Biology, 10: n/a. doi: 10.15252/msb.20145372
- Issue published online: 1 JUL 2014
- Article first published online: 1 JUL 2014
Variable screen quality, off-target effects, and unclear false discovery rates often hamper large-scale functional genomic screens in mammalian cells. Hart et al (2014) introduce gold standard reference sets of essential and non-essential genes, aiming at standardizing the analysis of genome-wide screens. This work provides a framework to compare both the quality and analysis methods of functional genetic screens.