Photothermal Monitoring of Redox State of Respiratory Chain in Single Live Cells

Authors


  • Posted on the web site on 22 February 2002.

*To whom correspondence should be addressed at: Luikov Heat and Mass Transfer Institute, 15, Brovka Street, Minsk 220072, Belarus. Fax: 375-172-842486; ld@hmti.ac.by

ABSTRACT

A method for monitoring respiratory chain (RC) activity in single live cells is described. It is based on the registration of the photothermal (PT) response after a laser pulse of a live single cell. The dependence of the PT-response amplitude and shape upon redox state of RC components was studied with a PT microscope for two in vitro models: (1) solutions of the RC component cytochrome c and (2) mice hepatocytes. The parameters of the PT responses differed for oxidized and reduced forms of cytochrome c solutions and for inhibited RC and intact RC. The latter difference may be caused by alteration of the quantum yields of thermal (nonradiative) relaxation for light-absorbing molecules, i.e. RC components, as they undergo reduction during RC inhibition.

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