Get access

Ultraviolet B but Not A Radiation Activates Suppressor B Cells in Draining Lymph Nodes

Authors

  • Scott N. Byrne,

    1. Department of Medicine, Dermatology Research Laboratories, Melanoma and Skin Cancer Research Institute, Sydney Cancer Centre, Royal Prince Alfred Hospital at the University of Sydney, Sydney, Australia
    Search for more papers by this author
  • Jarin Ahmed,

    1. Department of Medicine, Dermatology Research Laboratories, Melanoma and Skin Cancer Research Institute, Sydney Cancer Centre, Royal Prince Alfred Hospital at the University of Sydney, Sydney, Australia
    Search for more papers by this author
  • Gary M. Halliday

    Corresponding author
    1. Department of Medicine, Dermatology Research Laboratories, Melanoma and Skin Cancer Research Institute, Sydney Cancer Centre, Royal Prince Alfred Hospital at the University of Sydney, Sydney, Australia
    Search for more papers by this author

*To whom correspondence should be addressed: Department of Dermatology, Blackburn Building, D06, University of Sydney, Sydney, NSW, 2006, Australia. Fax: 61 2 9036 5130; e-mail: garyh@med.usyd.edu.au

ABSTRACT

Immunosuppressive doses of solar-simulated UV radiation activate lymph node B cells that can suppress primary immunity by inhibiting the function of dendritic cells. The aim of this study was to determine the waveband responsible for activation of these suppressor B cells. We exposed C57BL/6 mice to various doses of either UVA or UVB radiation and analyzed the number and activation state of lymph node antigen-presenting cells (APC). Immunosuppressive doses of UVB but not UVA activated B cells as assessed by major histocompatibility complex II (MHC II) expression and doubled their numbers in draining lymph nodes. Higher doses of UVA that were not immunosuppressive actually suppressed B cell activation. Our results show that UVA and UVB suppress systemic immunity via different mechanisms. Lymph node B cells are activated in response to immunosuppressive doses of UVB but not UVA. Thus, the activation state of lymph node APC appears to be important for UV immunomodulation.

Ancillary