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Distribution and Translocation of Photoreceptor Gβγ-phosducin System in Medaka Retina

Authors

  • Shintaro Yamamoto,

    1. Department of Earth and Space Science, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan
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    • Current address: Shintaro Yamamoto, Venture Business Laboratory, Center for Advanced Science and Innovation, Osaka University, Suita, Osaka, Japan.

  • Osamu Hisatomi,

    Corresponding author
    1. Department of Earth and Space Science, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan
      *email: hisatomi@ess.sci.osaka-u.ac.jp (Osamu Hisatomi)
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  • Akiyuki Hasegawa,

    1. Department of Earth and Space Science, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan
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  • Yuko Kobayashi,

    1. Department of Earth and Space Science, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan
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  • Fumio Tokunaga

    1. Department of Earth and Space Science, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan
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  • This invited paper is part of the Symposium-in-Print: Photobiology in Asia.

*email: hisatomi@ess.sci.osaka-u.ac.jp (Osamu Hisatomi)

Abstract

Unlike other vertebrates, teleosts have rod- and cone-specific phosducins (PD-R and PD-C) in the retina. To evaluate the teleost Gβγ-PD systems, we isolated cDNAs encoding medaka Gβ1 and GβC, which selectively expressed rods and cones. Immunohistochemical studies showed that the strong reactivity of GβC but not PD-C was detected in cone outer segments. In rod outer segments (ROS), PD-R reactivity was stronger in light-adapted retina than in dark-adapted retina. Western blot analyses of fractions torn from the cryosections showed that the PD-R concentration was low in dark-adapted ROS. It is suggested that PD-R is translocated to ROS and effectively downregulates the phototransduction cascade in light-adapted rods.

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