This invited paper is part of the Symposium-in-Print: DNA Photodynamics.
Direct Detection of 8-Oxo-Deoxyguanosine Using UV Resonance Raman Spectroscopy†
Article first published online: 5 JUN 2007
Photochemistry and Photobiology
Volume 83, Issue 3, pages 600–602, May/June 2007
How to Cite
Kundu, L. M. and Loppnow, G. R. (2007), Direct Detection of 8-Oxo-Deoxyguanosine Using UV Resonance Raman Spectroscopy. Photochemistry and Photobiology, 83: 600–602. doi: 10.1562/2006-04-15-RA-876
- Issue published online: 5 JUN 2007
- Article first published online: 5 JUN 2007
- Received 15 April 2006; accepted 16 June 2006; published online 29 June 2006
8-oxo-deoxyguanosine (8-oxo-dG) is a major oxidative lesion in DNA and is responsible for mutation and cancer. Current techniques for detecting 8-oxo-dG are indirect methods. Thus, development of new methodologies is needed to directly detect such oxidative lesions. In this article, we have used ultraviolet resonance Raman (UVRR) spectroscopy as a novel analytical technique for the detection of 8-oxo-dG. Here, the UVRR spectrum of 8-oxo-dG was acquired and compared to that of deoxyguanosine (dG) and deoxyadenosine (dA). Data analysis shows a distinct UVRR spectrum of 8-oxo-dG with characteristic peaks. Detection of 8-oxo-dG was easily achieved from a mixture with dG. These results reveal that UVRR spectroscopy shows promise as a direct method for detecting 8-oxo-dG.