Ultrafast Dynamics of Photoactive Yellow Protein via the Photoexcitation and Emission Processes

Authors

  • Ryosuke Nakamura,

    Corresponding author
    1. JST-CREST, Venture Business Laboratory, Center for Advanced Science and Innovation, Osaka University, Suita, Osaka 565-0871, Japan
      *Corresponding author email: ryo@mls.eng.osaka-u.ac.jp (Ryosuke Nakamura)
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  • Norio Hamada,

    1. JST-CREST, Venture Business Laboratory, Center for Advanced Science and Innovation, Osaka University, Suita, Osaka 565-0871, Japan
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  • Hideki Ichida,

    1. JST-CREST, Venture Business Laboratory, Center for Advanced Science and Innovation, Osaka University, Suita, Osaka 565-0871, Japan
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  • Fumio Tokunaga,

    1. JST-CREST, Department of Earth & Space Science, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan
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  • Yasuo Kanematsu

    1. JST-CREST, Venture Business Laboratory, Center for Advanced Science and Innovation, Osaka University, Suita, Osaka 565-0871, Japan
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  • This paper is part of the Proceedings of the 12th International Conference on Retinal Proteins held at Awaji Island, Hyogo, Japan on 4–8 June 2006.

*Corresponding author email: ryo@mls.eng.osaka-u.ac.jp (Ryosuke Nakamura)

Abstract

Pump-dump fluorescence spectroscopy was performed for photoactive yellow protein (PYP) at room temperature. The effect of the dump pulse on the population of the potential energy surface of the electronic excited state was examined as depletion in the stationary fluorescence intensity. The dynamic behavior of the population in the electronic excited state was successfully probed in the various combinations of the pump-dump delay, the dump-pulse wavelength, the dump-pulse energy and the observation wavelength. The experimental results were compared with the results obtained by the femtosecond time-resolved fluorescence spectroscopy.

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