Rapid Chlorophyll a Fluorescence Transients of Lemna minor Leaves as Indication of Light and Exogenous Electron Carriers Effect on Photosystem II Activity

Authors

  • D. Dewez,

    1. Department of Chemistry-TOXEN, University of Quebec in Montreal, Succ. Centre-Ville, Montreal, QC, Canada
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  • P. Eullaffroy,

    1. Laboratory of Plants, Pesticides and Sustainable Development, URVVC, University of Reims Champagne-Ardenne, Reims Cedex, France
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    • Current address: Environment Canada, Saint-Lawrence Center, Montreal, QC, Canada.

  • R. Popovic,

    1. Department of Chemistry-TOXEN, University of Quebec in Montreal, Succ. Centre-Ville, Montreal, QC, Canada
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  • P. Juneau

    Corresponding author
    1. Department of Biological Sciences-TOXEN, Canadian Research Chair on Ecotoxicology of Aquatic Microorganisms, University of Quebec in Montreal, Succ. Centre-Ville, Montreal, QC, Canada
      *e-mail: juneau.philippe@uqam.ca (P. Juneau)
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*e-mail: juneau.philippe@uqam.ca (P. Juneau)

Abstract

By using saturating flash, we investigated the change in the rapid fluorescence rise when Lemna minor leaf was exposed to different light conditions and treated with exogenous electron acceptors (methyl viologen and duroquinone) and electron donor (hydroxylamine). Investigation was carried out by using combined pulse amplitude modulated fluorometer and plant efficiency analyzer system, which were employed simultaneously to provide different light conditions and to induce rapid fluorescence rise respectively. We have shown that when leaf of L. minor was exposed to different conditions of illumination, rapid fluorescence rise was greatly influenced by the electron transport functions beyond quinone A–plastoquinone reduction. This was indicated by the change in both fluorescence yield and appearance time of the different transients. When exogenous electron donor (hydroxylamine) and acceptors (methyl viologen and duroquinone) were applied in in vivo condition, we showed that rapid fluorescence rise represented a reliable indicator of PSII–PSI electron transport state and energy dissipation process.

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