Current address: Environment Canada, Saint-Lawrence Center, Montreal, QC, Canada.
Rapid Chlorophyll a Fluorescence Transients of Lemna minor Leaves as Indication of Light and Exogenous Electron Carriers Effect on Photosystem II Activity
Article first published online: 27 FEB 2007
Photochemistry and Photobiology
Volume 83, Issue 3, pages 714–721, May/June 2007
How to Cite
Dewez, D., Eullaffroy, P., Popovic, R. and Juneau, P. (2007), Rapid Chlorophyll a Fluorescence Transients of Lemna minor Leaves as Indication of Light and Exogenous Electron Carriers Effect on Photosystem II Activity. Photochemistry and Photobiology, 83: 714–721. doi: 10.1562/2006-08-08-RA-999
- Issue published online: 9 MAY 2007
- Article first published online: 27 FEB 2007
- Received 3 August 2006; accepted 21 October 2006; published online 24 October 2006
By using saturating flash, we investigated the change in the rapid fluorescence rise when Lemna minor leaf was exposed to different light conditions and treated with exogenous electron acceptors (methyl viologen and duroquinone) and electron donor (hydroxylamine). Investigation was carried out by using combined pulse amplitude modulated fluorometer and plant efficiency analyzer system, which were employed simultaneously to provide different light conditions and to induce rapid fluorescence rise respectively. We have shown that when leaf of L. minor was exposed to different conditions of illumination, rapid fluorescence rise was greatly influenced by the electron transport functions beyond quinone A–plastoquinone reduction. This was indicated by the change in both fluorescence yield and appearance time of the different transients. When exogenous electron donor (hydroxylamine) and acceptors (methyl viologen and duroquinone) were applied in in vivo condition, we showed that rapid fluorescence rise represented a reliable indicator of PSII–PSI electron transport state and energy dissipation process.