• 1
    Williams DA. Ex vivo expansion of hematopoietic stem and progenitor cells—robbing Peter to pay Paul. Blood 1993; 81:31693172.
  • 2
    Bhatia M, Bonnet D, Kapp U, et al. Quantitative analysis reveals expansion of human hematopoietic repopulating cells after short-term ex vivo culture. J Exp Med 1997; 186:619624.
  • 3
    Traycoff CM, Kosak ST, Grigsby S, et al. Evaluation of ex vivo expansion potential of cord blood and bone marrow hematopoietic progenitor cells using cell tracking and limiting dilution analysis. Blood 1995; 85:20592068.
  • 4
    Gan OI, Murdoch B, Larochelle A, et al. Differential maintenance of primitive human SCID-repopulating cells, clonogenic progenitors, and long-term culture-initiating cells after incubation on human bone marrow stromal cells. Blood 1997; 90:641650.
  • 5
    Tsuji T, Ogasawara H, Aoki Y, et al. Characterization of murine stromal cell clones established from bone marrow and spleen. Leukemia 1996; 10:803812.
  • 6
    Kawada H, Ando K, Tsuji T, et al. Rapid ex vivo expansion of human umbilical cord hematopoietic progenitors using a novel culture system. Exp Hematol 1999; 27:904915.
  • 7
    Lapidot T, Pflumio F, Doedens M, et al. Cytokine stimulation of multilineage hematopoiesis from immature human cells engrafted in SCID mice. Science 1992; 255:11371141.
  • 8
    Vormoor J, Lapidot T, Pflumio F, et al. Immature human cord blood progenitors engraft and proliferate to high levels in severe combined immunodeficient mice. Blood 1994; 83:24892497.
  • 9
    Lansdorp PM, Dragowska W. Maintenance of hematopoiesis in serum-free bone marrow cultures involves sequential recruitment of quiescent progenitors. Exp Hematol 1993; 21:13211327.
  • 10
    Young JC, Varma A, DiGiusto D, et al. Retention of quiescent hematopoietic cells with high proliferative potential during ex vivo stem cell culture. Blood 1996; 87:545556.
  • 11
    Matsunaga T, Kato T, Miyazaki H, et al. Thrombopoietin promotes the survival of murine hematopoietic long-term reconstituting cells: comparison with the effects of FLT3/FLK-2 ligand and interleukin-6. Blood 1998; 92:452461.
  • 12
    Lansdorp PM, Dragowska W, Mayani H. Ontogeny-related changes in proliferative potential of human hematopoietic cells. J Exp Med 1993; 178:787791.
  • 13
    Terstappen LW, Huang S, Safford M, et al. Sequential generations of hematopoietic colonies derived from single nonlineage-committed CD34+CD38 progenitor cells. Blood 1991; 77:12181227.
  • 14
    Petzer AL, Hogge DE, Lansdorp PM, et al. Self-renewal of primitive human hematopoietic cells (long-term-culture-initiating cells) in vitro and their expansion in defined medium. Proc Natl Acad Sci USA 1996; 93:14701474.
  • 15
    Bhatia M, Wang JCY, Kapp U, et al. Purification of primitive human hematopoietic cells capable of repopulating immune-deficient mice. Proc Natl Acad Sci USA 1997; 94:53205325.
  • 16
    Zandstra PW, Conneally E, Piret JM, et al. Ontogeny-associated changes in the cytokine responses of primitive human haemopoietic cells. Br J Haematol 1998; 101:770778.
  • 17
    Larochelle A, Vormoor J, Hanenberg H, et al. Identification of primitive human hematopoietic cells capable of repopulating NOD/SCID mouse bone marrow: implications for gene therapy. Nat Med 1996; 2:13291337.
  • 18
    Yonemura Y, Ku H, Hirayama F, et al. Interleukin 3 or interleukin 1 abrogates the reconstituting ability of hematopoietic stem cells. Proc Natl Acad Sci USA 1996; 93:40404044.
  • 19
    Matsunaga T, Hirayama F, Yonemura Y, et al. Negative regulation by interleukin-3 (IL-3) of mouse early B-cell progenitors and stem cells in culture: transduction of the negative signals by βc and βIL-3 proteins of IL-3 receptor and absence of negative regulation by granulocyte-macrophage colony-stimulating factor. Blood 1998; 92:901907.
  • 20
    Tisdale JF, Hanazono Y, Sellers SE, et al. Ex vivo expansion of genetically marked rhesus peripheral blood progenitor cells results in diminished long-term repopulating ability. Blood 1998; 92:11311141.
  • 21
    Guenechea G, Segovia JC, Albella B, et al. Delayed engraftment of nonobese diabetic/severe combined immunodeficient mice transplanted with ex vivo-expanded human CD34(+) cord blood cells. Blood 1999; 93:10971105.
  • 22
    Traycoff CM, Kosak ST, Grigsby S, et al. Evaluation of ex vivo expansion potential of cord blood and bone marrow hematopoietic progenitor cells using cell tracking and limiting dilution analysis. Blood 1995; 85:20592068.
  • 23
    Srour EF, Tong J, Eder P, et al. Numbers of human CD34+ hematopoietic progenitor cells mobilized into peripheral blood with high-dose cyclophosphamide and recombinant cytokines increase in vitro following a short exposure to stem cell factor. Exp Hematol 1993; 21:1184.
  • 24
    Dexter TM, Allen TD, Lajtha LG. Conditions controlling the proliferation of haemopoietic stem cells in vitro. J Cell Physiol 1977; 91:335344.
  • 25
    Gartner S, Kaplan HS. Long-term culture of human bone marrow cells. Proc Natl Acad Sci USA 1980; 77:47564759.
  • 26
    Breems DA, Blokland EA, Siebel KE, et al. Stroma-contact prevents loss of hematopoietic stem cell quality during ex vivo expansion of CD34+ mobilized peripheral blood stem cells. Blood 1998; 91:111117.
  • 27
    Bennaceur-Griscelli A, Tourino C, Izac B, et al. Murine stromal cells counteract the loss of long-term culture-initiating cell potential induced by cytokines in CD34+CD38low/neg human bone marrow cells. Blood 1999; 94:529538.
  • 28
    Moore KA, Pytowski B, Witte L, et al. Hematopoietic activity of a stromal cell transmembrane protein containing epidermal growth factor-like repeat motifs. Proc Natl Acad Sci USA 1997; 94:40114016.