research-article
You have full text access to this OnlineOpen article
Differentiation of Human Embryonic Stem Cells into Corneal Epithelial-Like Cells by In Vitro Replication of the Corneal Epithelial Stem Cell Niche
Article first published online: 25 JAN 2007
DOI: 10.1634/stemcells.2006-0516
Copyright © 2007 AlphaMed Press
Additional Information
How to Cite
Ahmad, S., Stewart, R., Yung, S., Kolli, S., Armstrong, L., Stojkovic, M., Figueiredo, F. and Lako, M. (2007), Differentiation of Human Embryonic Stem Cells into Corneal Epithelial-Like Cells by In Vitro Replication of the Corneal Epithelial Stem Cell Niche. STEM CELLS, 25: 1145–1155. doi: 10.1634/stemcells.2006-0516
Publication History
- Issue published online: 2 JAN 2009
- Article first published online: 25 JAN 2007
- Manuscript Accepted: 15 JAN 2007
- Manuscript Received: 18 AUG 2006
References
- 1, . Establishment in culture of pluripotential cells from mouse embryos. Nature 1981; 292:154–156.
- 2, , et al. Embryonic stem cell lines derived from human blastocysts. Science 1998; 282:1145–1147.
- 3, , et al. Derivation of human embryonic stem cells from day-8 blastocysts recovered after three-step in vitro culture. Stem Cells 2004; 22:790–797.
- 4, , et al. Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: Implications on epithelial stem cells. Cell 1989; 57:201–209.
- 5. The epithelial basement membrane zone of the limbus. Eye 1989; 3:132–140.
- 6, , et al. Human corneal basement membrane heterogeneity: Topographical differences in the expression of type iv collagen and laminin isoforms. Lab Invest 1995; 72:461–473.
- 7, . Three patterns of cytokine expression potentially involved in epithelial-fibroblast interactions of human ocular surface. J Cell Physiol 1995; 163:61–79.
- 8, . Identification and characterization of limbal stem cells. Exp Eye Res 2005; 81:247–264.
- 9, , et al. Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers. Mol Med 2000; 6:88–95.
- 10. The stem cell system. Biomed Pharmacother 1983; 37:375–380.
- 11, . Normal stem cells and cancer stem cells: The niche matters. Cancer Res 2006; 66:4553–4557.
- 12, , et al. Osteoblastic cells regulate the haematopoietic stem cell niche. Nature 2003; 425:841–846.
- 13, , et al. Identification of the haematopoietic stem cell niche and control of the niche size. Nature 2003; 425:836–841.
- 14, , et al. Bg01v: A variant human embryonic stem cell line which exhibits rapid growth after passaging and reliable dopaminergic differentiation. Restor Neurol Neurosci 2004; 22:421–428.
- 15, , et al. Human embryonic stem cell-derived cd34+ cells: Efficient production in the coculture with op9 stromal cells and analysis of lymphohematopoietic potential. Blood 2005; 105:617–626.
- 16, , . Generation of lymphohematopoietic cells from embryonic stem cells in culture. Science 1994; 265:1098–1101.
- 17, , et al. Differentiation of human embryonic stem cells into hematopoietic cells by coculture with human fetal liver cells recapitulates the globin switch that occurs early in development. Exp Hematol 2005; 33:1450–1458.
- 18, , . Collagens in ocular tissues. Br J Ophthalmol 1993; 77:515–524.
- 19, , . Collagens and collagen-related matrix components in the human and mouse eye. Prog Retin Eye Res 2004; 23:403–434.
- 20, , et al. Basement membrane components in healing rabbit corneal epithelial wounds: Immunofluorescence and ultrastructural studies. J Cell Biol 1984; 98:128–138.
- 21, , . Coordinated appearance of beta 1 integrins and fibronectin during corneal wound healing. J Lab Clin Med 1992; 120:86–93.
- 22, , et al. Induction of epithelial progenitors in vitro from mouse embryonic stem cells and application for reconstruction of damaged cornea in mice. Invest Ophthalmol Vis Sci 2004; 45:4320–4326.
- 23. Derivation of keratinocyte progenitor cells and skin formation from embryonic stem cells. Int J Dev Biol 2004; 48:203–206.
- 24, , et al. Reconstituted skin from murine embryonic stem cells. Curr Biol 2003; 13:849–853.
- 25, , . Marker succession during the development of keratinocytes from cultured human embryonic stem cells. Proc Natl Acad Sci U S A 2003; 100:15625–15630.
- 26, , et al. Differentiation of embryonal stem cells into keratinocytes: Comparison of wild-type and beta 1 integrin-deficient cells. Dev Biol 1996; 179:184–196.
- 27, , et al. Proteoglycan synthesis by bovine keratocytes and corneal fibroblasts: Maintenance of the keratocyte phenotype in culture. Invest Ophthalmol Vis Sci 1999; 40:1658–1663.
- 28, , et al. An autogeneic feeder cell system that efficiently supports growth of undifferentiated human embryonic stem cells. Stem Cells 2005; 23:306–314.
- 29, , et al. Formation of pluripotent stem cells in the mammalian embryo depends on the pou transcription factor oct4. Cell 1998; 95:379–391.
- 30, , et al. Functional expression cloning of nanog, a pluripotency sustaining factor in embryonic stem cells. Cell 2003; 113:643–655.
- 31, , et al. The homeoprotein nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell 2003; 113:631–642.
- 32, , et al. Surface antigens of human embryonic stem cells: Changes upon differentiation in culture. J Anat 2002; 200:249–258.
- 33, , et al. Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 2002; 20:329–337.
- 34, , et al. P63 identifies keratinocyte stem cells. Proc Natl Acad Sci U S A 2001; 98:3156–3161.
- 35, , . Differentiation-related expression of a major 64k corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. J Cell Biol 1986; 103:49–62.
- 36, , . Expression of the 55-kd/64-kd corneal keratins in ocular surface epithelium. Invest Ophthalmol Vis Sci 1990; 31:448–456.
- 37, , et al. Cytoplasmic localization of p63 is associated with poor patient survival in lung adenocarcinoma. Histopath 2006; 49:349–357.
- 38, , et al. Unique gene expression signatures of independently-derived human embryonic stem cell lines. Hum Mol Genet 2004; 13:601–608.
- 39, , et al. Unique gene expression signature by human embryonic stem cells cultured under serum-free conditions correlates with their enhanced and prolonged growth in an undifferentiated stage. Stem Cells 2006; 24:151–167.
- 40, , et al. Gene expression signatures of seven individual human embryonic stem cell lines. Stem Cells 2005; 23:1343–1356.
- 41, , et al. The role of PI3K/AKT, MAPK/ERK and NFkappabeta signalling in the maintenance of human embryonic stem cell pluripotency and viability highlighted by transcriptional profiling and functional analysis. Hum Mol Genet 2006; 15:1894–1913.
- 42, , et al. A bivalent chromatin structure marks key developmental genes in embryonic stem cells. Cell 2006; 125:315–326.
- 43, , et al. Characterization of the expression of mhc proteins in human embryonic stem cells. Proc Natl Acad Sci U S A 2002; 99:9864–9869.
- 44, , et al. Banking on human embryonic stem cells: Estimating the number of donor cell lines needed for HLA matching. Lancet 2005; 366:2019–2025.
- 45, , . Genetic manipulation of human embryonic stem cells: A system to study early human development and potential therapeutic applications. Curr Gene Ther 2005; 5:375–385.

1549-4918/asset/olbannerleft.jpg?v=1&s=699114e871887e6b838f6a1c657fe256cfe127a6)
1549-4918/asset/olbannerright.gif?v=1&s=603f8f2ab5cd9d4f783c231915608956af51aeea)
1549-4918/asset/cover.gif?v=1&s=51ac62b4272cd760b70f0cb1539035cc40743da6)