Functional Expression of Ion Channels in Mesenchymal Stem Cells Derived from Umbilical Cord Vein
Article first published online: 24 MAY 2007
Copyright © 2007 AlphaMed Press
Volume 25, Issue 8, pages 2044–2052, August 2007
How to Cite
Park, K. S., Jung, K. H., Kim, S. H., Kim, K. S., Choi, M. R., Kim, Y. and Chai, Y. G. (2007), Functional Expression of Ion Channels in Mesenchymal Stem Cells Derived from Umbilical Cord Vein. STEM CELLS, 25: 2044–2052. doi: 10.1634/stemcells.2006-0735
- Issue published online: 2 JAN 2009
- Article first published online: 24 MAY 2007
- Manuscript Accepted: 11 MAY 2007
- Manuscript Received: 13 NOV 2006
- Mesenchymal stem cells;
- Voltage-gated K+ currents;
- Tetrodotoxin-sensitive Na+ current;
- Umbilical cord vein
Mesenchymal stem cells have the ability to renew and differentiate into various lineages of mesenchymal tissues. We used undifferentiated human mesenchymal-like stem cells from human umbilical cord vein (hUC-MSCs), a cell line which contains several mesenchymal cell markers. We characterized functional ion channels in cultured hUC-MSCs with whole-cell patch clamp and reverse transcription-polymerase chain reaction (RT-PCR). Three types of outward current were found in these cells: the Ca2+-activated K+ channel (IKCa), a transient outward K+ current (Ito), and a delayed rectifier K+ current (IKDR). IKCa and IKDR were totally suppressed by tetraethylammonium, and IKCa was sensitive to a specific blocker, iberiotoxin. Ito was inhibited by 4-aminopyridine. Another type of inward rectifier K+ current (Kir) was also detected in approximately 5% of hUC-MSCs. Elevation of external potassium ion concentration increased the Kir current amplitude and positively shifted its reversal potential. In addition, inward Na+ current (INa) was found in these cells (∼30%); the current was blocked by tetrodotoxin and verapamil. In the RT-PCR analysis, Kv1.1, Kv4.2, Kv1.4, Kir2.1, heag1, MaxiK, hNE-Na, and TWIK-1 were detected. These results suggested that multiple functional ion channel currents, IKCa, IKDR, Ito, INa, and Kir, are expressed in hUC-MSCs.
Disclosure of potential conflicts of interest is found at the end of this article.