Concise Review: Trends in Stem Cell Proteomics

Authors

  • Hossein Baharvand Ph.D.,

    Corresponding author
    1. Department of Stem Cells, Royan Institute, Tehran, Iran
    • Department of Stem Cells, Royan Institute, P.O. Box 19395-4644, Tehran, Iran. Telephone: 98-21-22172330; Fax: 98-21-22414532
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  • Ali Fathi,

    1. Department of Stem Cells, Royan Institute, Tehran, Iran
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  • Dennis van Hoof,

    1. Hubrecht Laboratory, Netherlands Institute of Developmental Biology, Utrecht, The Netherlands
    2. Department of Biomolecular Mass Spectrometry, Utrecht University, Utrecht, The Netherlands
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  • Ghasem Hosseini Salekdeh Ph.D.

    Corresponding author
    1. Department of Physiology and Proteomics, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran
    • Physiology and Proteomics Department, Agricultural Biotechnology Research Institute of Iran (ABRII), P.O. Box 31535-1897, Karaj, Iran. Telephone: 0098-261-2702893; Fax: 98-261-2704539
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Abstract

Gene expression analyses of stem cells (SCs) will help to uncover or further define signaling pathways and molecular mechanisms involved in the maintenance of self-renewal, pluripotency, and/or multipotency. In recent years, proteomic approaches have produced a wealth of data identifying proteins and mechanisms involved in SC proliferation and differentiation. Although many proteomics techniques have been developed and improved in peptide and protein separation, as well as mass spectrometry, several important issues, including sample heterogeneity, post-translational modifications, protein-protein interaction, and high-throughput quantification of hydrophobic and low-abundance proteins, still remain to be addressed and require further technical optimization. This review summarizes the methodologies used and the information gathered with proteome analyses of SCs, and it discusses biological and technical challenges for proteomic study of SCs.

Disclosure of potential conflicts of interest is found at the end of this article.

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