β-Catenin Signaling Promotes Proliferation of Progenitor Cells in the Adult Mouse Subventricular Zone
Article first published online: 2 AUG 2007
Copyright © 2007 AlphaMed Press
Volume 25, Issue 11, pages 2827–2836, November 2007
How to Cite
Adachi, K., Mirzadeh, Z., Sakaguchi, M., Yamashita, T., Nikolcheva, T., Gotoh, Y., Peltz, G., Gong, L., Kawase, T., Alvarez-Buylla, A., Okano, H. and Sawamoto, K. (2007), β-Catenin Signaling Promotes Proliferation of Progenitor Cells in the Adult Mouse Subventricular Zone. STEM CELLS, 25: 2827–2836. doi: 10.1634/stemcells.2007-0177
- Issue published online: 2 JAN 2009
- Article first published online: 2 AUG 2007
- Manuscript Accepted: 23 JUL 2007
- Manuscript Received: 19 MAR 2007
- Cell proliferation;
- Subventricular zone;
- Olfactory bulb;
The subventricular zone (SVZ) is the largest germinal zone in the mature rodent brain, and it continuously produces young neurons that migrate to the olfactory bulb. Neural stem cells in this region generate migratory neuroblasts via highly proliferative transit-amplifying cells. The Wnt/β-catenin signaling pathway partially regulates the proliferation and neuronal differentiation of neural progenitor cells in the embryonic brain. Here, we studied the role of β-catenin signaling in the adult mouse SVZ. β-Catenin-dependent expression of a destabilized form of green fluorescent protein was detected in progenitor cells in the adult SVZ of Axin2-d2EGFP reporter mice. Retrovirus-mediated expression of a stabilized β-catenin promoted the proliferation of Mash1+ cells and inhibited their differentiation into neuroblasts. Conversely, the expression of Dkk1, an inhibitor of Wnt signaling, reduced the proliferation of Mash1+ cells. In addition, an inhibitor of GSK3β promoted the proliferation of Mash1+ cells and increased the number of new neurons in the olfactory bulb 14 days later. These results suggest that β-catenin signaling plays a role in the proliferation of progenitor cells in the SVZ of the adult mouse brain.
Disclosure of potential conflicts of interest is found at the end of this article.