Noninvasive Tracking of Cardiac Embryonic Stem Cells In Vivo Using Magnetic Resonance Imaging Techniques
Version of Record online: 9 AUG 2007
Copyright © 2007 AlphaMed Press
Volume 25, Issue 11, pages 2936–2944, November 2007
How to Cite
Ebert, S. N., Taylor, D. G., Nguyen, H.-L., Kodack, D. P., Beyers, R. J., Xu, Y., Yang, Z. and French, B. A. (2007), Noninvasive Tracking of Cardiac Embryonic Stem Cells In Vivo Using Magnetic Resonance Imaging Techniques. STEM CELLS, 25: 2936–2944. doi: 10.1634/stemcells.2007-0216
- Issue online: 2 JAN 2009
- Version of Record online: 9 AUG 2007
- Manuscript Accepted: 31 JUL 2007
- Manuscript Received: 26 MAR 2007
- Cell transplantation;
- In vivo tracking;
- Embryonic stem cells;
Despite rapid advances in the stem cell field, the ability to identify and track transplanted or migrating stem cells in vivo is limited. To overcome this limitation, we used magnetic resonance imaging (MRI) to detect and follow transplanted stem cells over a period of 28 days in mice using an established myocardial infarction model. Pluripotent mouse embryonic stem (mES) cells were expanded and induced to differentiate into beating cardiomyocytes in vitro. The cardiac-differentiated mES cells were then loaded with superparamagnetic fluorescent microspheres (1.63 μm in diameter) and transplanted into ischemic myocardium immediately following ligation and subsequent reperfusion of the left anterior descending coronary artery. To identify the transplanted stem cells in vivo, MRI was performed using a Varian Inova 4.7 Tesla scanner. Our results show that (a) the cardiac-differentiated mES were effectively loaded with superparamagnetic microspheres in vitro, (b) the microsphere-loaded mES cells continued to beat in culture prior to transplantation, (c) the transplanted mES cells were readily detected in the heart in vivo using noninvasive MRI techniques, (d) the transplanted stem cells were detected in ischemic myocardium for the entire 28-day duration of the study as confirmed by MRI and post-mortem histological analyses, and (e) concurrent functional MRI indicated typical loss of cardiac function, although significant amelioration of remodeling was noted after 28 days in hearts that received transplanted stem cells. These results demonstrate that it is feasible to simultaneously track transplanted stem cells and monitor cardiac function in vivo over an extended period using noninvasive MRI techniques.
Disclosure of potential conflicts of interest is found at the end of this article.