Pleiotrophin Enhances Clonal Growth and Long-Term Expansion of Human Embryonic Stem Cells
Article first published online: 6 SEP 2007
Copyright © 2007 AlphaMed Press
Volume 25, Issue 12, pages 3029–3037, December 2007
How to Cite
Soh, B. S., Song, C. M., Vallier, L., Li, P., Choong, C., Yeo, B. H., Lim, E. H., Pedersen, R. A., Yang, H. H., Rao, M. and Lim, B. (2007), Pleiotrophin Enhances Clonal Growth and Long-Term Expansion of Human Embryonic Stem Cells. STEM CELLS, 25: 3029–3037. doi: 10.1634/stemcells.2007-0372
- Issue published online: 2 JAN 2009
- Article first published online: 6 SEP 2007
- Manuscript Accepted: 23 AUG 2007
- Manuscript Received: 15 MAY 2007
- Transcriptome database;
- Feeder cells and human embryonic stem cells;
- Growth factors and receptors Clonal efficiency and embryonic stem expansion
To identify additional growth factors for optimizing propagation of human embryonic stem cells (hESCs), we mined publicly available data sets for the transcriptomes of murine and human ESCs and feeder cells, thereby generating a list of growth factors and complementary receptors. We identified the major pathways previously reported to be important, as well as several new ones. One pathway is the Pleiotrophin (PTN)-Pleiotrophin receptor (PTPRZ1) axis. Murine fibroblasts secrete Ptn, whereas hESCs expressed PTPRZ1, which is downregulated upon differentiation. Depletion of PTPRZ1 resulted in decreased colony formation and lower recovery of hESCs. Supplementation of chemically defined medium for feeder-free propagation of hESCs with PTN allowed higher recovery of hESCs without loss of pluripotency. PTN-PTPRZ1 functions here predominantly via an antiapoptotic effect mediated in part by the activation of Akt. These findings reveal the underlying importance of PTN in hESC survival and its usefulness in the clonal manipulation and large-scale propagation of hESCs.
Disclosure of potential conflicts of interest is found at the end of this article.