The Stem Cell Marker Prominin-1/CD133 on Membrane Particles in Human Cerebrospinal Fluid Offers Novel Approaches for Studying Central Nervous System Disease
Article first published online: 20 DEC 2007
Copyright © 2008 AlphaMed Press
Volume 26, Issue 3, pages 698–705, March 2008
How to Cite
Huttner, H. B., Janich, P., Köhrmann, M., Jászai, J., Siebzehnrubl, F., Blümcke, I., Suttorp, M., Gahr, M., Kuhnt, D., Nimsky, C., Krex, D., Schackert, G., Löwenbrück, K., Reichmann, H., Jüttler, E., Hacke, W., Schellinger, P. D., Schwab, S., Wilsch-Bräuninger, M., Marzesco, A.-M. and Corbeil, D. (2008), The Stem Cell Marker Prominin-1/CD133 on Membrane Particles in Human Cerebrospinal Fluid Offers Novel Approaches for Studying Central Nervous System Disease. STEM CELLS, 26: 698–705. doi: 10.1634/stemcells.2007-0639
- Issue published online: 2 JAN 2009
- Article first published online: 20 DEC 2007
- Manuscript Accepted: 11 DEC 2007
- Manuscript Received: 6 AUG 2007
- Human hematopoietic stem cells;
- Nervous system;
- Somatic stem cells
Cerebrospinal fluid (CSF) is routinely used for diagnosing and monitoring neurological diseases. The CSF proteins used so far for diagnostic purposes (except for those associated with whole cells) are soluble. Here, we show that human CSF contains specific membrane particles that carry prominin-1/CD133, a neural stem cell marker implicated in brain tumors, notably glioblastoma. Differential and equilibrium centrifugation and detergent solubility analyses showed that these membrane particles were similar in physical properties and microdomain organization to small membrane vesicles previously shown to be released from neural stem cells in the mouse embryo. The levels of membrane particle-associated prominin-1/CD133 declined during childhood and remained constant thereafter, with a remarkably narrow range in healthy adults. Glioblastoma patients showed elevated levels of membrane particle-associated prominin-1/CD133, which decreased dramatically in the final stage of the disease. Hence, analysis of CSF for membrane particles carrying the somatic stem cell marker prominin-1/CD133 offers a novel approach for studying human central nervous system disease.
Disclosure of potential conflicts of interest is found at the end of this article.